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MatTek/EpiDermFT™/epidermft/1 Ea
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Thedevelopmentofinnovativeskincareproductsrequiresadvanced,human-relevantinvitrotestingtechnologies.WhenworldleADIngpersonalcareandcosmeticscientistsevaluatenovelingredientsandformulations,theychoosetheEpiDermFT3Dhumanskinmodel.LargeDonorInventorywithMatchedKeratinocytesandFibroblastsAdultorNeonatalDonorsareAvailableDeliveredReady-to-UseTechnology:Engineeredtoenableinvitroskinresearchinwhichfibroblast-keratinocytecellinteractionsareimportant,MatTek’sEpiDermFTsystemconsistsofnormal,humanepidermalkeratinocytes(NHEK)andnormal,humandermalfibroblasts(NHFB)culturedtoformamultilayeredmodelofthehumandermisandepidermis.Culturedattheair-liquidinterfaceineasy-to-handletissuecultureinserts,EpiDermFTattainslevelsofdifferentiationatthecuttingedgeoftissueengineeringtechnology.EpiDermFTconsistsoforganizedkeratin5expressingbasalcells,involucrinandkeratin10expressingspinousandgranularlayers,andcornifiedepidermallayersanalogoustothosefoundinvivo.Thedermalcompartmentiscomposedofacollagenmatrixcontainingviablenormalhumandermalfibroblasts(NHDF).Theepidermalanddermallayersaremitoticallyandmetabolicallyactiveandexhibitinvivo-likemorphologicalandgrowthcharacteristicswhichareuniformandhighlyreproducIBLe.Keratin5Keratin10InvolucrinAwell-developedbasementmembraneispresentatthedermal/epidermaljunction.Hemidesomosomes(›),laminalucida,laminadensa(→)andanchoringfibrilstructures(»)areevidentbytransmissionelectronmicroscopy.Applications:EpiDermFTtissuesareusedacrossawiderangeofapplicationsincludingscientificefficacyandclaimssubstantiation.Duetoadryskinsurface,EpiDermFTcanbeusedtoevaluateBIOLOGicalresponsestotopicalapplicationsofformulationsoringredients.Simpleprotocolsandtheevaluationofearlymolecularendpointsallowresearchtoacquiredataindays,notweeksormonths.Anti-AgingExaminechangesinextracellularmatrixgeneandproteinexpressionandhistologicalstructuretodeterminetheanti-agingefficacyofcosmeticingredientsorfinalformulation.EpiDermFTisavailablefromarangeofadultdonors.Anti-AgingApplicationNoteWoundHealingUseEpiDermFTtoevaluatetherapeuticswiththewoundhealingassaykit.Woundhealingassaykitsinclude24pre-woundedtissuesandareavailablewithepidermalonlywoundsorfullthicknesswoundsoftheepidermisanddermis.RecentWoundHealingPosterSkinHydrationUseEpiDermFTtoassesstheefficacyoftopicallyappliedmoisturizersbymeasuringtheelectricalimpedanceoftheepidermis.Amenabletocreams,gel,lotionsorotherformulations,theSkinHydrationassayallowsfordataanalysiswithin24hours.SkinHydrationApplicationNoteUVProtectionAnalyzecyclopyrimidinedimerformation,inflammatorymediatorreleaseandoxidativeendpointstoevaluatetheUVprotectiveefficacyofcompoundsorformulations.UVProtection ApplicationNoteBrowseourreferencelibrarytoseehowourEpiDermFT tissuehasbeenusedintheseareasofstudy.a[rel~="mtli_filesize82534kB"]:after {content:" (825.34 kB)"}a[rel~="mtli_filesize60666kB"]:after {content:" (606.66 kB)"}a[rel~="mtli_filesize48179kB"]:after {content:" (481.79 kB)"}a[rel~="mtli_filesize81959kB"]:after {content:" (819.59 kB)"}TechSpecs:TissueKit: AstandardEpiDermFTkit(EFT-400)consistsof24tissues.(Tissue“kits”containtissues,asmallamountofculturemedium,andplasticware;contactMatTekforspecifickitcontents)Substrate: CostarSnapwell™singlewelltissuecultureplateinserts areused.Poresize=0.4μm,Diameter=1.2cm.Surfacearea=1.0cm2Culture: Air-liquidinterfaceHistology: 8-12celllayersplusstratumcorneum(basal,spinous,andgranularlayers)Lotnumbers: Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualkitswithinagivenlotoftissues.Alltissuekitswithinalotareidenticalinregardstocells,medium,handling,cultureconditions,etc.Shipment: At4°Conmedium-supplemented,agarosegelsin6-wellplatesShipmentday: EveryMonday.ShipmentonThursdayalsopossibleuponspecialrequestDelivery: TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-ThursdaydependingonlocationShelflife: Includingtimeintransit,tissuesmaybestoredat4°Cforupto6dayspriortouse.However,extendedstorageperiodsarenotrecommendedunlessnecessary.Inaddition,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning)Lengthofexperiments: Culturescanbecontinuedforupto2weekswithgoodretentionofnormalepidermalmorphology.Culturesmustbefedeveryotherdaywith5.0mlofEFT-400medium CellsType: Normalhumanepidermalkeratinocytes(NHEK);Normalhumandermalfibroblasts(NHDF)Geneticmake-up: SingledonorDerivedfrom: Neonatal-foreskintissue(NHEK);Neonatalskin(NHDF)Alternatives: NHEKfromadultbreastskin;NHDFfromadultskinScreenedfor: HIV,Hepatitis-B,Hepatitis-C,mycoplasma MediumBasemedium: Dulbecco’sModifiedEagle’sMedium(DMEM)Growthfactors/hormones: Epidermalgrowthfactor,insulin,hydrocortisoneandotherproprietarystimulatorsofepidermaldifferentiationSerum: NoneAntibiotics: Gentamicin5µg/ml(10%ofnormalgentamicinlevel)Anti-fungalagent: AmphotericinB0.25µg/mlpHIndicator: PhenolredOtheradditives: Lipidprecursorsusedtoenhanceepidermalbarrierformation(proprietary)Alternatives: Phenolred-free(EFT-400-PRF),antibiotic-free(EFT-400-ABF),anti-fungal-free(EFT-400-AFF),orhydrocortisone-freemediumandtissue(EFT-400-HCF)areavailable.Agentsareremovedatleast3dayspriortoshipmentAssay/Maintenancemedium: EFT-400-ASYisutilizedforassays;EFT-400-MMisusedfortermmaintenanceoftheEFT-400tissues QualityControlandSterilityVisualinspection: Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipmentEnd-usetesting: Tissuesareexposedto1%TritonX-100for8,12,16and24hours.ThetimeofexposurerequiredtoreducethetissueviABIlity(ET-50)usingtheMTTviabilityassayisdetermined(SeeMatTekEpiDermFTuseprotocol)foreachlotoftissue.ET-50’sgenerallyfallwithintherangeof6.5-9.5hours.ET-50’sincustomers’labmaydifferslightlyfromtheMatTekresultsSterility: Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontaminationScreeningforpathogens: AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“Biosafetyinmicrobiologicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalserviceNotificationoflotfailure: IfatissuelotfailsourQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutchargewhenappropriate.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossible(Undernormalcircumstances,ET-50failureswillbenotifiedbyWednesday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)

MatTek公司是麻省理工学院两个化学工程教授于1985成立的科技公司。1991年,该公司利用其核心的聚合物表面改性技术进入新兴的组织工程领域,目前已成长为的组织工程技术公司,致力于生产创新性的3D重构人体组织模型,该模型相较于传统2D细胞模型具有更可靠的实验结果。其生产的重构皮肤,眼部和呼吸道组织模型已广泛应用于化妆品,化工,医药和家用产品等行业的毒理学监控(OECD,欧盟的指导方针)及毒理学研究。


MatTek公司的基于人体细胞的3D皮肤模型EpiDerm1993年研发上市,立即获得巨大的成功。从此MatTek通过以下方式彻底改变了基于人体细胞的体外模型市场:(1)低成本(降低50-80%的成本);(2)通过发表质量控制标准(同时具备阳性和阴性对照)以确保可为行业及监管机构接受的高可重复性水平;(3)优化研究人员的实验结果。通过创造性使用非动物的体外测试,融合降低(直接/间接)成本的强大协同效应及显著提高的产品质量,MatTek极大的降低产品成本、提高实验室效率及高质量的高可重复性产品,减少实验动物的使用,具有无可超越的长期组织可重复性;3-D仿真结构;高度分化的组织模型;代谢和分裂活性;源于正常的人体细胞;培养、操作简便;无血清培养;可定量的客观检测;物美价廉的优点。因而使得MatTek人细胞来源的体外组织模型被包括美国、加拿大、欧洲、日本和东亚的众多跨国公司与研究机构采用。


目前MatTek公司的产品主要包括EpiDerm(皮肤模型)、MelanoDerm(黑色素模型)、EpiOcular(眼周表皮模型)、EpiAirway呼吸道上皮模型)、EpiDermFT (全厚的皮肤模型EpiVaginal(阴道上皮模型)、EpiOral(口腔上皮模型)、 EpiGingivalTM(牙龈上皮模型)、Psoriasis tissue(牛皮癣组织模型)及树突细胞。


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