MatTek’s3DtissuemodelofthehumansmallintestineisourlatestinnovationadvancinginvitroGIresearchworldwide.Allowingforphysiologicalexposuresandhuman-relevantendpoints,EpiIntestinalisbeingincorporatedintopharmaceuticaldevelopmentprogramsacrosstheglobe.EpithelialPolarityHighlyDifferentiated,PhysiologicallyRelevantMultipleDonorsAvailableDeliveredReady-to-use&Easy-to-useTechnology: TissueCultureInsertTheEpiIntestinaltissuemodel(SMI-100)offersnumerousadvantagesversuscurrentlyavailablepreclinicaltestingmodelssuchasCaco-2.Thehumancell-based3Dmodelincorporatesenterocytes,panethcell,Mcells,tuftcellsandintestinalstemcellsintoahighlydifferentiated, polarizedepithelium.TheEpiIntestinalFTtissue(SMI-100-FT)includesanunderlyinglaminapropriacontainingnormalhumanintestinalfibroblastsandisusefultostudyinflammation,woundrepair,innateimmuneresponses,andfibrosis.Bothtissuesareculturedattheair-liquidinterface(ALI)toallowphysiological(luminal)exposureconditions.EpiIntestinalrecapitulates manyaspectsofnormalintestinalfunctionincluding barrier,metabolism,inflammatoryandtoxicityresponses,similartonativehumanintestinaltissue.ImportantfeaturesofEpiIntestinalincludethepresenceoffunctionaltightjunctionsandbrushbordersattheapicaltissuesurface. Invivo,brushbordersareindirectcontactwithluminalcontentsandincludeatleast22metabolicenzymesand19drugtransporters. EpiIntestinaltightjunctionsandbrushborderscloselymimichumanstructureandphysiology. Applications:TheEpiIntestinal3DhumantissuemodelisroutinelyutilizedforscreeningpharmaceuticalsforarangeofendpointsincludingGItoxicityandinflammation,drugmetabolism,drugpermeation,fibrosis,andintestinalinfection.Straightforwardprotocolsandtheevaluationofearlymolecularandcellularendpointsoftenallowscientiststoacquiredataindays,notweeksormonths.GIToxicityUseEpiIntestinalforahighlypredictiveassaytoevaluateGItoxicityofpharmaceuticals.GItoxicityreferenceInflammationandFibrosisUtilizeEpiIntestinal’shighlycontrolledinvitrocultureconditionstostudyintestinalinflammation,woundrepair,andfibrosis andassesstherapeuticeffects.IntestinalInflammationReferenceDrugDelivery&MetabolismUtilizeEpiIntestinalforhighlypredictiveandhuman-relevantdrugdelivery. AssessAPIbioavailABIlity(influx)andeffluxtransport,drugmetabolism,anddrug-druginteractions. EpiIntestinalcanbeco-culturedwithotherphysiologicalsystems(e.g.livercells)inmicrofluidicsystemstoexaminetheeffectofdrugmetabolitesonGItoxicityortopredictdrugpermeation/absorptionviathe“luminal”sideofthetissue.IntestinalDrugDeliveryReferenceWoundRepair/EpithelialRestitutionUseEpiIntestinalFTtobetterunderstandmechanismsofwoundhealinginthegutandtoevaluatetherapeuticeffects.IntestinalRestitutionReferenceIntestinalInfectionUtilizeEpiIntestinaltostudyGItractinfection,host-pathogeninteractions,andinnateimmuneresponses.BrowseourreferencelibrarytolearnmoreabouthowresearchershaveusedourEpiIntestinaltissueintheseareasofstudy. a[rel~="mtli_filesize96797kB"]:after {content:" (967.97 kB)"}a[rel~="mtli_filesize118MB"]:after {content:" (1.18 MB)"}TechSpecs:Tissue:Kit:SMI-100andSMI-100-FTEpiIntestinalkitsconsistof24individualtissues. SMI-196orSMI-196-FTEpiIntestinalkitsconsistof96tissuesinahighthroughputscreeningplate. Eachkit containstissues,culturemediumtosupportexperimentsfor24hours,andplasticware. PleasecontactMatTekforspecifickitcontents.Formats:SMI-100andSMI-100-FT: Individualtissuesculturedincellcultureinserts.Dimensions: 9mmtissuediameter(surfacearea=0.6cm2),underlyingmicroporousmembraneporesize= 0.4μmSMI-196andSMI-196-FT: Tissuesgrownin96-wellhighthroughputscreeningplate. Dimensions: 0.19cmtissuediameter(surfacearea=0.11cm2). Underlyingmicroporousmembraneporesize=0.4μmCulture:Air-liquidinterfaceLotnumbers:Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualsetsof24tissueswithinagivenproductionlotoftissues.Alltissuekitswithinaproductionlotareidenticalinregardstocells,medium,handling,cultureconditions,etc.Shipment:At4°Conmedium-supplemented,agarosegelsShipmentday:EveryMondayDelivery:TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-Wednesday dependingonlocation.Shelflife:Includingtimeintransit,tissuesmaybestoredat4°Cforupto2dayspriortouse.However,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning).Lengthofexperiments:TissueculturescanbecontinuedforONE(1)MONTHormorewithgoodretentionofnormalmorphology.Tissuesmustbefedeveryotherdaywith5.0mlofmaintenancemedium(SMI-100-MM).Cellcultureinsertsareplacedatopwashers(EPI-WSHR)orculturestands(MEL-STND)in6-wellplatestoallowuseof5.0ml.Cells:Type:Primaryhumansmallintestinalepithelialcells(HSIE)Geneticmake-up:SingledonorDerivedfrom:HealthyadultdonorAlternatives:AlternatedonorsavailableuponrequestScreenedfor:HIV,Hepatitis-B,Hepatitis-C,mycoplasmaMedium:Basemedium:Dulbecco’sModifiedEagle’sMedium(DMEM)Growthfactors/hormones:EpidermalgrowthfactorandotherproprietaryfactorsAntibiotics:Gentamicin5µg/mlAnti-fungalagent:AmphotericinB0.25µg/mlpHIndicator:PhenolredOtheradditives:ProprietaryAlternatives:Phenolred-free,antibiotic-free,anti-fungal-freemediumandtissuesareavailable.Agentsareremovedatleast3dayspriortoshipment.QualityControlandSterility:Visualinspection:Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipmentSterility:Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontaminationScreeningforpathogens:AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“BiosafetyinmicroBIOLOGicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalserviceNotificationoflotfailure:IfatissuelotfailsQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutcharge.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossIBLe(Undernormalcircumstances,ET-50failureswillbenotifiedbyThursday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)