Description:T4 RNA Ligase 1 catalyzes the ligation of a 5\'-phosphoryl-terminated nucleic acid donor to a 3\'-hydroxyl-terminated nucleic acid acceptor through the formation of a 3\' -5\' phosphodiester bond, with hydrolysis of ATP to AMP and PP_i. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.Application:- Labeling of 3\'-termini of RNA with 5\'-[³²P] pCp- Inter- and intramolecular joining of RNA and DNA molecules- Synthesis of single-stranded oligodeoxyribo-nucleotides- Incorporation of unnatural amino acids into proteins- Ligation of ssRNA and DNASource:An E. coli strain that carries the T4 RNA Ligase 1 gene.1x T4 RNA Ligase Reaction Buffer (and 10mM ATP):50 mM Tris-HCl10 mM MgCl₂1 mM DTTpH 7.5 @ 25°CHeat Inactivation:65°C for 15 minutesHeat inactivation by boiling for 2 minutesReaction Conditions:1x T4 RNA Ligase Reaction BufferSupplemented with 1 mM ATPIncubate at 37°CUnit Definition:One unit is defined as the amount of enzyme required to convert 1 nmol of 5´-[³²P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.Unit Assay Conditions:1x T4 RNA Ligase reaction buffer, supplemented with 1 mM ATP, is mixed with the RNA substrate (10µM of 5\'-[³²P]rA16 ) and varying amounts of enzyme. Incubation is at 37°C for 15 minutes.Concentration: 10,000 units/ml

Specific Activity: 168,000 U/mgStorage Conditions:10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25°CRecommended Storage Condition: -20°C