Description:Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides, while not binding well to double-stranded DNA. In nature, SSB participates in DNA replication, recombination, and repair functions. In vitro, SSB has been found to stimulate certain DNA polymerase-mediated reactions by relaxing DNA secondary structure and enhancing enzyme processivity.Application:- effective in fluorescence polarization assays- eliminates pausing when sequencing through strong secondary structures- helps obtain longer read lengths in pyrosequencing for SNP analysisSource:A recombinant E. coli strain carrying the E. coli SSB gene.Supplied in:50 mM Tris-HCl200 mM NaCl1.0 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25°CRecommended Storage Condition: -20°C |