Description:Endonuclease IV (E. coli) is a class II apurinic/apyrimidic (AP) enzyme that cleaves 5\' to an AP site by hydrolysis, leaving a hydroxyl group at the 3\' terminus and a deoxyribose 5\'-phosphate at the 5\'-terminus. Endo IV can be used in vivo to repair free radical damage in DNA.Application:- DNA structure research- Studies of DNA damage and repair- Single cell electrophoresis (comet assay)- Anti-tumor drug studiesSource:E. coli cells with a cloned nfo gene.Unit Definition:One unit of the enzyme relaxes 1µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 min at 37°CStorage Buffer:Supplied in 50% Glycercol, 50 mM Tris-HCl (pH 7.5), 1mM DTT, 0.1 M NaCl, 0.1% Triton X-10010x Endonuclease IV Buffer1M NaCl500 mM Tris-HCl100 mM MgCl₂10 mM DTT pH 7.9 @ 25°CQuality Control:Endonuclease IV is tested in degradation of dsDNA and is free of detectable RNase and double-stranded exonuclease activities.Recommended Storage Condition: -20°CReference:1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711.2. Levin, J.D. and Demple, B. (1996) Nucleic Acids Res. 24, 885.