I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification from different templates including plasmids, BACS, genomic DNA, and lambda DNA.
General Description I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification of from different templates including plasmids, BACS, genomic DNA, and lambda DNA. It allows for amplification of up to 8kb with human genomic DNA and up to 21kb with lambda DNA. It has an extension speed of 1 kb / 10-15 seconds depending on template type. This allows the user to save time by speeding up PCR reactions and provides higher fidelity than Taq or Pfu.Feature • Robust – maximal success and minimal optimization needed• Fidelity – 50X greater than Taq• High Speed –10X faster than Pfu• High Yield• Versatile – best for long or difficult templatesSourceE. coli Applications PCRCloningLong or Difficult AmplificationHigh-Throughput PCR Supplied with 5X Reaction Buffer (1ml), 50mM MgCl2 (1ml) Supplied in (buffer description)20mM Tris-HCl, 100mM KCl, 1mM DTT, 0.1mM EDTA, 200ug/ml BSA, 50% glycerol, 1X stabilizer, pH 7.4 @ 25 C Storage Condition -20°C Unit Definition One unit is the amount of enzyme that incorporates 5 nmol of dNTPs into acid insoluble material in 15 minutes at 72°C. References Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.Shipping Condition dry ice |
