Description:Phi29 DNA Polymerase is responsible for the replication of the Bacillus Subtilis phage phi29⁽¹⁾. The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity⁽²⁾ and a 3\' -> 5\' proofreading exonuclease function⁽³⁾.Specific Activity: 100,000U/mgApplication:- Catalyzes the removal of 5\'-mononucleotides from duplex DNA- Replication requiring a high degree of strand displacement and/or processive synthesis- High fidelity replication at moderate temperaturesSource:A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.Supplied in:10 mM Tris-HCl100 mM KCl0.1 mM EDTA1 mM DTT0.5% Tween-200.5% NP-4050% GlycerolpH 7.4 @ 25°CSupplied with:10x phi29 DNA Polymerase Reaction Buffer10x phi29 DNA Polymerase Buffer500 mM Tris-HCl100 mM (NH₄)₂SO₄40 mM DTT100 mM MgCl₂pH 7.5 @ 25°CUnit Definition:1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.Recommended Storage Condition: -20°CReferences:1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329.2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940.3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599.