Description:Lambda Exonuclease is a highly processive exonuclease which selectively degrades the 5\'-phosphorylated strand of double-stranded DNA via the stepwise 5\' to 3\' release of mononucleotides from duplex DNA. Lambda Exonuclease is inactive against 5\'-hydroxyl termini⁽¹⁾, and will not initiate excision at a nick or gap⁽²⁾, though it will degrade a 5\'-overhanging tail from duplex DNA at a greatly reduced rate.Specific Activity: 100,000 U/mgApplication:Catalyzes the removal of 5\'-mononucleotides from duplex DNA.Source:Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.Supplied in:25 mM Tris-HCl50 mM NaCl1.0 mM DTT0.1% mM EDTA50% GlycerolpH 7.5 @ 25°CSupplied with:10x Lambda Exo Reaction Buffer10x Lambda Exo Reaction Buffer:670 mM Glycine25 mM MgCl₂500 μg/ml BSApH 9.4 @ 25°CUnit Definition:One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrates in 30 minutes at 37°C.Recommended Storage Condition: -20°CReferences:1. Ausubel, F. M.,et al. (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.)2. Little, J.W. (1981) Gene Amp. Anal., 2, 135-145