Description:Catalyses the oxidation of luciferin to oxyluciferin in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8):Application:- Assesses the transcriptional activity in cells- Detects the level of cellular ATP in cell viability assaysSource:E. coli recombinant enzyme prepared from a thermostable mutant.Unit Definition:One light unit produces a biometer peak height equivalent to 0.02 µCi of 14C in PPO/POPOP cocktail. Light units measured in 50 µl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.Storage Conditions:10 mM Tris-HCl pH 7.8100 mM NaCl0.1 mM DTT0.1 mM EDTA50% GlycerolHeat Stability:Active at 42°C for at least 24 hours (with < 5% loss of activity)Recommended Storage Condition: -20°CReference:Kricka UJ. (1988) \"Clinical and biochemical applications of luciferases and luciferins\", Anal Biochem. 175 (1): 14-21