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MCLAB/McExtract Gel Extraction Kit/EGEK-150/1 Ea
MCLAB’s McExtract Gel Extraction Kit offers specially designed paramagnetic beads and buffers for rapid purification of DNA fragments from agarose gels.

Description MCLABs McExtract Gel Extraction Kit offers specially designed paramagnetic beads and buffers for rapid purification of DNA fragments from agarose gels. DNA ranging from 70 bp to 10 kb is extracted from molten gels using a rapid binding and magnetically separating procedure. Captured DNA is then washed and eluted. The procedure provides faster and easier way of DNA gel purification through magnetic beads and magnets rather than the use of column centrifuge. It can be easily transitioned to large-scale purification or automation.

Applications

DNA purified with the Gel Extraction Kit can be used directly in most molecular biology applications, including:

  • Subcloning
  • Sequencing
  • Labeling & DNA hybridization
  • DNA concentration

Highlights

  • Fast and easy purification of DNA fragments from an agarose gel using MCLABs magnetic beads
  • No need for centrifugation
  • A single kit fits from small to large scale of extractions, and can be easily adapted to an automation format

Experimental Data

 

Figure 1: Comparison of DNA fragments from agarose gel extracted with McExtract Gel Extraction Kit and competitor Qs kit  

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Gel on the left:L: 1 Kb ladder1: DNA product before gel purification MC: 2 kb DNA purified with McExtract Gel Extraction KitQ: 2 kb DNA purified with competitor Qs kitGel on the right:L: 1 Kb ladder1: DNA product before gel purificationMC: 1 kb DNA purified with McExtract Gel Extraction KitQ: 1 kb DNA purified with competitor Qs kit

Kit Component

Reagent/Size

Small size-MCGE150

Large size-MCGE500

McExtract

50 ml x 1 ea (~150 rxn/100mg gel slice)

155 ml x 1 ea (~500 rxn/100mg gel slice)