Description:Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5\'-phosphoryl and 3\'-hydroxyl termini, using NAD⁺ as a cofactor.Figure:Figure, Comparing to another vendor\'s product, the activity of MCLAB\'s Taq DNA ligase was tested with BstEII-digested Lamda DNA under 45°C for 15 minutes.Application:- Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction- Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplificationSource:A recombinant E. coli strain carrying the cloned Taq DNA Ligase gene.Supplied in:10 mM Tris-HCl50 mM KCl1 mM Dithiothreitol0.1 mM EDTA0.1% TWEEN 2050% GlycerolpH 7.4 @ 25°CSupplied with:10x Taq DNA Ligase Buffer10x Taq DNA Ligase Buffer:200 mM Tris-HCl250 mM Potassium Acetate100 mM Magnesium Acetate10 mM NAD 1100 mM DTT0.1% Triton® X-100pH 7.6 @ 25°C 

Unit Definition:

One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C.

Specific Activity: 400 Unit/µg

Recommended Storage Condition: -20°C