High purity α-Mannosidase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.24CAZy Family: GH38CAS: 9025-42-7
alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase
Recombinant. From Thermotoga maritima.In 3.2 M ammonium sulphate.Supplied at ~ 71 U/mL.
Specific activity: 4.5 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.0 and 90oC;~ 0.2 U/mg at 40oC.
Stability: > 4 years 4oC.
DESCRIPTION
α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima)
EC 3.2.1.24CAZy Family: GH38CAS: 9025-42-7
Synonyms:alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase
Form:In 3.2 M ammonium sulphate.
Stability:> 4 years 4oC.
Specific activity:4.5 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.0 and 90oC; ~ 0.2 U/mg at 40oC.
Unit definition:One Unit of α-D-Mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-mannopyranoside (2 mM) in MES buffer (100 mM) and CoCl2 (1 mM) at pH 6.0 at the temperatures indicated.
Specificity:Broad specificity; hydrolysis of terminal non-reducing alpha-D-mannose in alpha-D-mannosides.
Applications:For use in glycobiology research.
