产品

产品目录 > 常用试剂 > 蛋白酶

【名称】：Proteinase K, Tritirachium album/蛋白酶K 分装

【规格】：100MG

【货号】：539480-100MG/分

【品牌】：Merck/默克

【价格】：250

【说明书】：暂无

【储存和运输】：2度-8度。

Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK. Catalogue Number: 539480 Brand Family:Calbiochem Product Information: CAS number39450-01-6 Activity30 mAnsonU/mg dry weight Unit of DefinitionOne mAnson unit is defined as the amount of enzyme that will liberate 1.0 mol of Folin-positive amino acids (calculated as tyrosine) per min at 25 C, pH 7.5. EC number3.4.21.14 FormWhite lyophilized solid FormulationLyophilized from calcium acetate Molar Mass28500 Biological Information: Specific Activity40 mAnsonU/mg protein Physicochemical Information ContaminantsDNase: no detectable nicking activity with pBR322, incubation for 6 h at 37 RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37 C pH valueoptimum 7.5 - 12.0 Solubility50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 Safety Information: R PhraseR: 36/37/38-42 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation. S PhraseS: 22-24-26-36/37 Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. Storage and Shipping Information Ship CodeAmbient Temperature Only Toxicity Irritant Storage +2 C to +8 C Do not freezeOk to freeze Special InstructionsFollowing reconstitution, store in the refrigerator (4 C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4 C.