lumiprobe官网-蚂蚁淘生物

Lumiprobe, LLC.是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。主要产品有：活性染料（Reactive Dye）和SYBR Green I 染料，定制合成荧光双标记引物。产品主要应用：点击化学（Click Chemistry）、蛋白质组学研究中的双向荧光差异凝胶电泳（2D DIGE）和实时荧光定量PCR（Realtime PCR）。

花菁类染料-美国Lumiprobe

花菁这类染料的分子内部含有由甲川基(CH)n组成的共轭链，n可为奇数或偶数。共轭链两端或链中间连有杂环、芳环化合物、环烯化合物等与共轭链组成一个大的共轭体系，分子内部的氢可被一定数目的各类取代基取代。

基于其结构，花菁通常有非常高的超过100000 Lmol-1cm-1消光系数。不同的取代基具有控制发色团的性能，如吸收波长、耐光性、荧光。例如，通过聚次甲基桥键长度的选择来控制吸光度和荧光波长：聚次甲基桥键越长其吸光度越高且发射波长可达到近红外区。

已经有许多的花菁染料（Cyanine dyes）用于生命科学领域，一系列的噻唑和恶唑染料用作DNA 和蛋白质染色(如TOTO，YOYO， Stains All和其它)。但是在生命科学研究领域最受欢迎的花菁染料是由卡内基梅隆大学的Alan Waggoner和他的同事们在1990年代早期发现的。该花菁染料是经过修饰后的吲哚菁绿(ICG)，从1970年代以来该染料一直用于血管造影术，而且他们都包含两个假吲哚环侧翼(CH)n链。发现此类染料对生物分子表现出较低的非特异性结合，由于巨大的消光系数能发出明亮的荧光和良好的量子产量。一旦获得专利，在卡内基梅隆专利过期后这些分子可在公共领域获得，并从Lumiprobe可购买到，作为各种反应衍生物用于科研和商业，比如用于点击化学的NHS酯、马来酰亚胺、叠氮化物的衍生品和其他衍生物。

花菁染料分为两种：非磺化的花菁染料和磺化的花菁染料，对于许多的应用，他们是可以互相替换的，因为他们的光谱属性几乎相同。磺化和非磺化染料可用于DNA和蛋白质等生物分子的标记。两种染料的区别在于它们的溶解度：磺基染料是水溶性，他们可在水环境中标记无需添加有机助溶剂，他们在水里的分散性较好。在某些情况下，你可能会只需要这两种染料中的一种（下面将具体讲述非磺化的花菁染料和磺化的花菁染料的具体区别。）

非磺化的花菁染料

可用的非磺化的花菁染料包括Cy3，Cy3.5，Cy5，Cy5.5，Cy7，和Cy7.5，Cy代表‘cyanine’，第一个数字代表了假吲哚之间的碳原子数。Cy2是恶唑衍生物而不是假吲哚，是不遵守这个规则的。后缀.5是苯丙稠合的花菁染料。结构的变化也能改变分子的荧光特性，及覆盖最重要的可见光和携带几个荧光基团的近红外光谱。

大部分非磺化的花菁染料衍生物（酰肼盐酸盐类和胺类除外）具有低水溶性，当这些分子用于生物分子标记时，使用有机溶剂作为助溶剂(5-20%的DMF或DMSO)是有效的反应所必需的。花菁染料应先溶解在有机溶剂中，之后在适当的水溶缓冲剂作用下加入到生物分子溶液中（蛋白质，肽，氨基标记的 DNA）。在染料沉淀之前，染料分子与生物分子发生有效反应。

非磺化的花菁染料的荧光性质对溶剂和反应环境的依赖性较小，非磺化的的花菁染料的吸光度和荧光光谱绘制如下。

磺化的花菁染料

磺化的花菁染料包含了额外地磺基官能团能促进染料分子在水相中的溶解度，带电荷的磺酸盐降低了染料分子的聚合度且能很好地标记偶联物。

目前可用的磺化花菁染料包括sulfo-Cy3，sulfo-Cy5和sulfo-Cy7。

磺化花菁染料具有高度水溶性。标记生物分子时不需要有机助溶剂辅助。

磺化和非磺化花菁染料之间的异同

磺化和非磺化花菁染料都展现出非常相似的荧光性能。然而，在使用说明上有几个不同点需要注意，非磺化花菁染料必须要先溶解在有机助溶剂中（DMF or DMSO），之后在适当的水溶缓冲剂作用下加入靶标生物分子溶液。推荐使用的助溶剂的体积为，溶解Cy3， Cy5， Cy7使用10%助溶剂，.5的染料使用15%的助溶剂。而磺化的花菁染料可以在纯粹的水环境中应用。关于花菁染料在纯化方面的差异：当使用与水或水溶缓冲溶液相关的透析纯化染料时，必须使用sulfo-Cy以实现高效除去未反应的染料材料。与磺化和非磺化花菁染料的反应可以通过凝胶过滤，色谱法 (FPLC，高效液相色谱法，离子交换法)，或电泳纯化。

磺化和非磺化花菁染料在标记以下类别目标时是可互换的:

1.可溶性蛋白，其对额外添加的有机助溶剂具有耐受性

2.抗体(使用5 10% DMSO / DMF)

3.DNA和寡核苷酸

4.肽

5.许多小分子

注：用类似磺化和非磺化的试剂标记的共轭物（例如：sulfo-Cy5 和Cy5）的荧光性质是非常相似的，可用于各种荧光仪器。

以下情况必须选用磺化花菁染料：

1.遇DMF 或 DMSO变性的敏感蛋白质

2.通过透析纯化的蛋白质偶联物

3.在水溶液中有纳米粒子

4.不溶于水或疏水的蛋白

以下情况必须选用非磺化花菁染料：

在有机相中发生的反应（二氯甲烷，腈化甲烷）

This high load, 500 controlled pore glass solid support is designed for the synthesis of oligonucleotides containing up to 50 bases with a 3 -amino group. Amino group is protected with trifluoroacetyl (TFA) protection which is easily removed under standard deblock conditions.

The reagent is based on hydroxyprolinol core a universal non-nucleoside structure that is naturally 100% chiral (no isomers formed upon condensation), and stable to all conditions of oligonucleotide synthesis and deblock.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.

Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cyanine5 minimal dye for protein labeling, an analog of Cy5® minimal dye.This reagent is specially quantified for the use in 2D proteomics. Each package contains specified amount of NHS ester with quantity variation within 10%.

相关产品：Cyanine2 NHS ester minimal dyeCyanine3 NHS ester minimal dyeDMF, labeling grade solvent for reconstitution of dyes

Cy® is a trademark of GE Healthcare.Purchase of these products does not imply transfer of license to perform methods patented by Carnegie Mellon University covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 Difference gel electrophoresis using matched multiple dyes and family members in Australia, Canada, Europe and Japan.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Amino-11-dUTP is an improved replacement for Aminoallyl dUTP. This triphosphate features longer linker, which allows for easier and more facile downstream labeling.

Amino-11-dUTP is compatible with PCR, nick translation, and reverse transcription. It can be used for microarray labeling. The nucleotide is incorporated by Taq DNA polymerase, Klenow fragment, and reverse transcriptases. Subsequent reaction of amino-DNA with activated esters can be used for the labeling of DNA.

General properties

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5 , internal, or 3 position (using universal support).This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Biotin azide是生物素（维生素H）的衍生物，和叠氮基团共价结合。该试剂可以将多种炔烃化分子包括DNA，寡核苷酸，蛋白标记上生物素。生物素连接亲和素或链霉亲和素可以用于下游的亲和应用来分离生物素化分子或成为连接链霉亲和素的复合物。biotin azide的结构特点是拥有长的亲水链，可以在靶分子上将单独生物素显露出来用以亲和连接链霉亲和素。这种链（PEG）也增强了它的水溶性使得连接更易进行。这种叠氮分子可以和很多分子进行连接，可以参考我们的推荐手册。

Biotin azide

相关产品：

Biotin alkyne 物素衍生物，与点击化学反应互补，连接叠氮化物。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.

Ascorbic acid is the recommended reagent for the reduction of Copper(II)-TBTA complex into active Copper(I) form.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Biotin alkyne用于通过点击化学制备多种生物素化试剂。这种炔烃能和很多叠氮化物反应，包括含有叠氮基团的生物分子。生物素化标记可以用于很多靠亲和力连接的反应和应用。我们推荐的手册可以用于这种连接。Biotin alkyne

Biotin alkyne用于通过点击化学制备多种生物素化试剂。这种炔烃能和很多叠氮化物反应，包括含有叠氮基团的生物分子。生物素化标记可以用于很多靠亲和力连接的反应和应用。我们推荐的手册可以用于这种连接。推荐手册：

寡核苷酸与DNA的点击化学标记需要有机共溶剂（如DMSO）用于高效连接。

相关产品：

Biotin azide 点击化学的叠氮试剂，用于和炔烃进行连接。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.

Cyanine3 amine是功能化的花菁染料，包含游离的氨基基团。Cyanine3是Cy3®的类似物。该染料的氨基基团可以和NHS ester，羧基基团（碳二亚胺活化后）及环氧化合物等活性基团反应。

相关产品：

Cyanine5 amineCyanine5.5 amine

Cy® is a trademark of GE Healthcare.

General properties

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

High loading controlled pore glass solid support for the synthesis of oligonucleotides with 3 -alkyne group. With this solid support, synthesis of oligos of up to 50 bases is possible.Terminal alkyne group can be modified by copper catalyzed Click chemistry (see our recommended protocol for oligonucleotide modification).This solid support is compatible with standard oligonucleotide deblocking conditions. No special deblocking is required.

相关产品：

Alkyne Amidite, hydroxyprolinol alkyne phosphoramidite with DMT group for cartridge and HPLC purificationAlkyne Phosphoramidite, 5 -terminal solid alkyne phosphoramidite for 5 -terminal modification which is easy to handle and dispense

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Hydrazide derivative of Cyanine7, a NIR fluorescent dye, an analog of Cy7 .

This variety of heptamethine fluorophore contains a six-membered ring rigidizing the polymethine chain which allows to increase quantum yield by 20% compared to parent structure.

Hydrazide reacts quickly and smoothly with aldehydes and ketones yielding stable hydrazone products. This reaction is also good for the labeling of glycoproteins after periodate oxidation.

Cy is a trademark of GE Healthcare.

General properties

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as SYBR Green I or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Thiol reactive BDP FL maleimide是用于蛋白标记的活性染料，它和BODIPY® FL maleimide有着完全相同的结构。BDP FL的吸收和发射光谱和荧光素（FAM）相近。然而，这种染料却有着极好的耐光性，不带电，分子量较低。它的亮度和荧光素、R110、呫吨染料衍生的如Alexa Fluor® 488。这种荧光团很适用于荧光显微镜及其它应用。该荧光团可以在很多应用中替代荧光素，并适用于很多FAM兼容的仪器。

BDP FL maleimide

推荐手册：

蛋白与其他硫醇分子的马来酰亚胺标记

BODIPY® is a trademark of Life Technologies. Alexa Fluor® is a trademark of Invitrogen.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine3 NHS ester dye for protein labeling, and analog of Cy3® minimal dye.

This reagent is specially quantified each package contains specified amount of NHS ester with quantity variation within 10%.相关产品：

Cyanine2 NHS ester minimal dyeCyanine5 NHS ester minimal dyeDMF, labeling grade solvent for reconstitution of dyes

Cy® is a trademark of GE Healthcare.Purchase of these products does not imply transfer of license to perform mehtods patented by Carnegie Mellon University and covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 Difference gel electrophoresis using matched multiple dyes and family members in Australia, Canada, Europe and Japan.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Amino-11-ddUTP is a terminator triphosphate for enzymatic end-labeling of DNA. The nucleotide is incorporated by Taq DNA polymerase, Sequenase, Klenow fragment. Subsequent reaction of amino-terminated DNA with activated esters can be used for the end-labeling of DNA.

General properties

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

通过该试剂可以将你的蛋白和多肽转变为适合点击化学的活性形式。点击化学是包括末端炔烃和叠氮的反应，但在自然界中叠氮化物和炔烃却很罕见。但是，却有试剂可以将生物分子普遍含有的氨基基团连接上这种片段。这种azido-NHS ester就是为将蛋白、多肽、氨基-DNA和其他氨基转换为点击化学活性叠氮化物而设计的。Azidobutyric acid NHS ester

使用我们的标准氨基生物分子标记手册。

相关产品：

Pentynoic acid activated ester

General properties

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

水溶的炔烃活性酯试剂适合为多种生物分子比如蛋白和多肽连接炔烃基团。氨基-DNA及其他氨基亦适用。因为天然蛋白并不包含炔烃，但通常包含氨基（赖氨酸），该试剂就相当于\"转换器”将天然氨基分子转为炔烃。使用该试剂可以使所有蛋白适合点击化学。该试剂是STP （sulfotetrafluorophenyl）酯。STP酯类似NHS酯，但拥有更强的亲水性和水溶性。在不需要有机共溶剂单纯的水溶液中也能很好的进行标记反应，是对蛋白进行修饰的最好选择。

Pentynoic acid STP ester

推荐手册：使用我们的标准氨基生物分子标记手册。

相关产品：

Azidobutyric acid NHS ester 类似产品用于蛋白标记azido基团。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer.After the condensation of the trebler, three DNA branches begin to grow simultaneously with each step of the synthesis. Deblock of this construct gives rise to DNA containing branching point. One arm (stem) is attached to branch point with its 5 -end, and other arms (branches) are attached via 3 -end. Reverse amidites can be used to prepare constructs with different branch orientations. Repetitive condensations of trebler results in formation of DNA dendrimers.Trebler amidite can be used to attach several modifier amidites to 5 -end of oligonucleotide for example, three biotin residues can be attached at once.This amidite does not require any special handling. Recommended coupling time is 5 minutes. No changes to deblock conditions are required.

General properties

Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

DMF (dimethylformamide) is a recommended solvent for labeling reactions using NHS esters. However, when DMF is stored in air, it deteriorates slowly absorbing moisture, and releasing dimethylamine. These impurities rapidly destroy NHS ester function, decrease labeling efficiency, and even make labeling reaction fail.

Dimethylformamide solvent requires special drying and preparation, which is often not available in all laboratories.

This product contains ready-to use solvent which is guaranteed to be useable for the labeling. Tubes with high quality, dry, and amine-free DMF are packed under argon.

General properties

SYBR Green I是一种敏感的dsDNA结合染料，可以用于日常琼脂糖和聚丙烯酰胺凝胶的DNA检测。不同于Ethidium，SYBR Green I对双链DNA有高度选择性，更低的毒性，更高的灵敏度。

溴化乙锭（Ethidium Bromide）和SYBR Green I的对比

SYBR Green I DNA凝胶染色Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

A carbonyl-reactive hydrazide derivative of Cyanine7.5 near infrared (NIR) fluorophore. This dye has similar spectral properties with ICG (indocyanine green), but has a higher quantum yield of fluorescence. Hence, this fluorophore is ideal for in vivo imaging.

This is a carbonyl-reactive dye which is particularly useful for the labeling of antibodies after periodate oxidation. It also labels other carbonyl compounds (aldehydes and ketones), for example, many saccharides.

一种羰基活性酰肼衍生的Cyanine7.5近红外荧光团。这种染料和ICG（吲哚菁绿），但是有更高的荧光量子产率。因此，这种荧光团是活体成像的理想选择。

这种羰基活性染料对高碘酸氧化的抗体的标记尤其有效。它也可以用于羰基化合物（醛和酮），比如很多的糖类。

General properties

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine5.5 (Cy5.5®的类似物)的氨基衍生物。该染料包含游离的氨基基团，可以和很多功能化合物包括NHS esters和环氧化物反应。Cyanine5.5是远红外染料，可以很好的用于活体成像，和要求低荧光背景的反应。

相关产品：

Cyanine5 amine red-emitting amino-dye, with lower absorption and emission wavelengthsCyanine7 amine NIR dye with amine group

Cy® is a trademark of GE Healthcare.

General properties

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine2 minimal dye for protein labeling, an equivalent of Cy2® NHS ester minimal dye.

This reagent is specially quantified each package contains specified amount of NHS ester with quantity variation within 10%.

相关产品：Cyanine3 NHS ester minimal dyeCyanine5 NHS ester minimal dyeDMF, labeling grade solvent for reconstitution of dyes

Cy2® is a trademark of GE Healthcare.Purchase of these products does not imply transfer of license to perform mehtods patented by Carnegie Mellon University and covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 Difference gel electrophoresis using matched multiple dyes and family members in Australia, Canada, Europe and Japan.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

水溶的亲水的sulfo-Cyanine5 maleimide（Cy5® maleimide的类似物）。作为Cy5® maleimide的完美的替代物，我们推荐该产品用于蛋白、包括抗体的标记。通过凝胶过滤、离心柱纯化、透析、电泳或色谱层析方法就可以很容易的将标记蛋白和未标记的染料进行分类。没有磺化基团的类似物：Cyanine5 maleimide。

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Aminolink phosphoramidite for oligonucleotide synthesis. DMS(O)MT is a special protective group similar to traditional MMT, but designed as an improved alternative to it. DMS(O)MT aminolink is fully compatible with standard coupling, deblock, and purification protocols. However, DMS(O)MT group is much better suited for DMT-on and cartridge purification of amino-oligonucleotides because of higher stability, and better oligonucleotide recovery. Oligonucleotides containing DMS(O)MT are more stable in solution than MMT analogs.In case of cartridge purification, DMS(O)MT protection allows for 2-3 fold increase of oligonucleotide yield, making this reagent a unique solution for high-throughput manufacturing of amino-oligonucleotides.

General properties

Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.

Sulfo-Cyanine7羧酸是非活性染料，水溶的近红外染料。当不需要标记其他分子时该染料通常用作近红外范围的荧光标准。该染料拥有高度亲水和水相的溶解度，改进的近红外范围量子产率，非常高的摩尔消光系数。其包括游离羧酸功能团。查看活性染料酯。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

R110 (rhodamine 110) azide，有固体和10mM浓度溶于DMSO的溶液两种形式选择。用于点击化学的标记试剂。纯化的5-isomer.与荧光素的光物理性质类似，但更耐光。可以替代Alexa Fluor 488，DyLight 488和FAM (fluorescein)。R110 azide, 5- isomer

R110 (rhodamine 110) azide，有固体和10mM浓度溶于DMSO的溶液两种形式选择。用于点击化学的标记试剂。纯化的5-isomer.与荧光素的光物理性质类似，但更耐光。可以替代Alexa Fluor 488，DyLight 488和FAM (fluorescein)。推荐手册：

寡核苷酸与DNA的点击化学标记

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cy5.5 hydrazide是含羰基的近红外活性染料。酰肼可以与一些羰基化合物迅速反应，例如醛类和酮类，并能形成稳定的腙。该反应可用于标记偶联物的制备。

生物分子中的羰基基团可以在氧化应激或蛋白质的脱氨基作用之后自发的形成。或者，醛基可以通过糖蛋白分子糖残基的高碘酸氧化特异生成，例如抗体。抗体可以被高碘酸钠氧化，并且可以被Cy5.5酰肼标记，从而形成Cy5.5酰肼标记的抗体。由于抗体中的糖残基远离可变的表位结合位点，用这种方法标记抗体是很理想的。

Cy5.5是一种近红外荧光团，是Cy5®的类似物。它特别适用于对低荧光背景有要求的应用。该染料的吸收和发射波长具有极强的组织穿透力，所以Cy5.5可用于体内近红外成像应用。

相关产品：

Cyanine3 hydrazideCyanine5 hydrazideCy® is a trademark of GE Healthcare.

General properties

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Sulfo-Cyanine3 maleimide是水溶的，硫醇活性染料用于亲水sulfo-Cyanine3荧光团的标记。是Cy3® maleimide的类似物。该产品推荐用于温和的单纯的水环境中抗体和其他不稳定蛋白的标记。该染料是水溶的，因此不需要有机共溶剂。非磺化的Cyanine3 maleimide。

推荐手册：

蛋白与其他硫醇分子的马来酰亚胺标记

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

水溶的sulfo-Cyanine3 dye，非活性、单功能团的羧基染料。该试剂可以用作Cy3®检测通道的参照荧光团，作为使用其他sulfo-Cyanine3标记的产品的机器的对照。羧酸也可以用于碳化二亚胺活化。

其吸收和发射光谱和Cy3®荧光团是一样的。

相关产品：sulfo-Cyanine3 NHS ester 氨基标记活性酯sulfo-Cyanine5 carboxylic acid 水溶的sulfo-Cyanine5 carboxylic acid

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Standard fluorescein (FAM) phosphoramidite for 5 -terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers. Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

General properties

Storage: 12 months after receival at -20 C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.

该Cyanine5.5标记试剂是叠氮类染料，用于点击化学。有10mM 溶解于DMSO和粉剂两种形式可供选择。这种染料具有远红/近红外发射光，可以用于NIR活体成像。Cyanine5.5可以替代Cy5.5®，Alexa Fluor 680和DyLight 680。

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cyanine5 hydrazide是用于标记醛和酮的活性染料。是Cy5® hydrazide的类似物。该染料能够很容易与含羰基的分子以近乎理想化的程度反应。比如承受氧化压力的蛋白，糖基化的蛋白通过高碘酸氧化作用重新活化（包括抗体），含醛基的寡核苷酸。Cyanine5 hydrazide可以替代carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649等染料。

Cy® is a trademark of GE Healthcare.

General properties

significant solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

硫醇活性染料、近红外Cyanine7.5，用于标记蛋白的巯基基团，是Cy7.5® maleimide的类似物。大多数蛋白含有巯基基团，可以选择性的标记马来酰亚胺。该试剂可以使这些蛋白连接上近红外荧光团。然后，该复合物可以用于非入侵性的活体成像，来研究组织和生物体内的蛋白分布。

推荐手册：

蛋白与其他硫醇分子的马来酰亚胺标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

用于点击化学的包含alkyne基团的水溶染料。Sulfo-Cyanine5是磺化的，有很高的亲水性，水溶，相当高的消光系数，很好的量子产率，与很多仪器相兼容。该试剂推荐用于标记蛋白、纳米微球及其他亲水性应用。

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

sulfo-Cyanine5 azide azide derivative of the same dyesulfo-Cyanine3 azide water-soluble Cyanine3 azideCu(II)-TBTA Click Chemistry catalyst

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

未活化的Cyanine7近红外羧酸染料。如果需要偶联和标记反应，请参考使用活性染料Cyanine7.5 NHS ester。

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Phosphoramidite for the synthesis of oligonucleotides with 5 -terminal alkyne for Click Chemistry.This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5 -butynyl-CEP, and other 5 -terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf lifeDiluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5 -terminal DMT group, no 5 -deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommended protocol).相关产品：

Alkyne Amidite, hydroxyprolinol alkyne phosphoramidite with DMT group for cartridge and HPLC purification

General properties

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

Cyanine7 azide是近红外荧光染料，用于点击化学标记，是Cy7® azide的类似物。该产品可以用于点击化学中Cyanine7结合标记炔烃化生物分子。后续寡核苷酸的合成修饰已可以用这种叠氮类染料进行。环己烷桥连聚亚甲基链会较之前没有链式结构的量子产率提高20%。

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine3 hydrazide属于羰基活性染料，是Cy3® hydrazide的类似物。该试剂可以用于标记含有羰基的分子比如抗体及其他高碘酸盐氧化反应后的糖蛋白，承受氧化压力的蛋白或去氨基反应的蛋白，还原糖等。Cyanine3可以和很多荧光仪器相兼容。

相关产品：

Cyanine5 hydrazide

Cy® is a trademark of GE Healthcare.

General properties

significant solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

近红外染料，含有游离氨基基团可以活化酯和其他活性分子。 Cyanine7是近红外染料，是Cy7 的类似物，尤其适合活体成像及对低背景有要求的应用。

Cy is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

近红外、巯基活性染料，是Cy7® maleimide的类似物。该试剂可以用于含游离巯基基团的蛋白标记近红外Cyanine7。标记的蛋白可以用于近红外生物成像。近红外成像系统可以使组织甚至活体内标记蛋白的分布可视化。

推荐手册：

蛋白与其他硫醇分子的马来酰亚胺标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol).Diluent for this amidite is acetonitrile, 5 min coupling time is recommended. Standard deprotection conditions can be used for oligonucleotides.Oligonucleotides can be purified by HPLC or cartridges due to the presence of dimethoxytrityl group, as well as by PAGE.

相关产品：Alkyne Phosphoramidite, 5 -terminal 5 -terminal amidite which is easy to handle and dispense

General properties

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

用于点击化学标记的远红/近红外炔烃染料。Cyanine5.5是CY5.5的类似物，一种流行的荧光团，可以广泛的用于各种应用的标记，包括完整的生物成像。这种染料可以在点击化学温和的铜催化剂的条件下连接叠氮基团，这种染料溶于有机溶剂，但在有少量DMSO存在的水溶液中即可有效的进行标记。Cyanine5.5 alkyne可以用小分子的标记。

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

Cyanine5.5 azide 包含叠氮基团的同样的染料Cyanine5 alkyne 另一个发射红色荧光的alkyne染料Cy® is a trademark of GE Healthcare.

General properties

good in organic solvents (DMF, DMSO, acetonitrile, DCM, alcohols), low in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

FAM azide有两种形式可供选择，粉剂和10mM浓度溶于DMSO的溶液。用于点击化学的试剂标记。该产品是纯化的6-isomer。可以替代Alexa Fluor 488和DyLight 488。

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

FAM azide, 5-isomer

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cyanine5 amine是含有氨基基团的活性染料，是Cy5® amine的类似物。该试剂可以和很多活化酯和亲电子试剂相结合。比如，该氨基可以和EDC活化的羧基基团反应。这种高亮度的耐光的染料适合很多种不同的荧光检测试验。用肉眼即可很容易的观察到痕量（纳摩尔）的这种颜色丰富的荧光团。

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Boc保护的Cyanine5 hydrazide活性染料用于醛和酮的标记。Boc- (tert-butoxycarbonyl)保护基团可以通过三氟乙酸或其他强酸去除。活化的hydrazide形成后，便可以进行后续标记羰基基团的反应得到Cyanine5标记的分子。

相关产品：

Cyanine5 hydrazide, free deprotected hydrazide.

General properties

good in dichloromethane, polar organic solvents (DMF, DMSO), alcohols, low in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine5.5 maleimide是硫醇活性染料，能够选择性的雨蛋白的巯基基团进行标记，是Cy5.5® maleimide的类似物。Cyanine5.5的近红外发射光使得其适合可以用于生物成像。Cyanine5.5可以替代Cy5.5®，Alexa Fluor 680和DyLight 680。

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine5 alkyne用于点击化学，是CY5的类似物。使用这种产品，深颜色的，耐光的Cyanine5荧光团，可以通过点击化学反应对多种叠氮分子进行标记。该染料是非水溶的，可以在反应前在有机DMS或DMSO中溶解，然后加入水相反应混合液中。请参考我们的标记手册，即可进行有效的、高产率的标记反应。多种包含叠氮的基质都可以用于标记反应，包括生物分子，聚合物和粉剂表面。

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

BDP FL azide是BODIPY FL azide的类似物，可以用于点击化学的高亮度且对光稳定的荧光染料，用于荧光仪器的FAM通道。这种发射绿光的荧光染料能与所有荧光测量机器相兼容，类似于Alexa Fluor 488，用于FAM通道。

该荧光素是borondipyrromethene系列荧光染料的代表，在水环境中拥有较高的量子产率，而且对光稳定。

BODIPY and Alexa Fluor are trademarks of Life Technologies.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine5 maleimide是单一活性染料，有选择性的与硫醇基团（比如蛋白和多肽的半胱氨酸）结合以进行标记。我们推荐使用水溶的Sulfo-Cyanine5 maleimide标记抗体和其他敏感蛋白。Cyanine5是Cy5®的类似物，与很多仪器比如显微镜、成像仪、荧光读板机相兼容的常用荧光团。

推荐手册：蛋白与其他硫醇分子的马来酰亚胺标记

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine3 alkyn适用于点击化学反应的荧光团。有了这个明亮且耐光的Cyanine3 reporter试剂，CY3的衍生物，就可以对几乎任何包含叠氮基团的分子进行标记。这种炔烃非水溶的。然而，即使在有有机共溶剂（DMSO或DMF）的情况下，这种染料依然能有效的对水溶的分子如蛋白、多肽进行标记。

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

Cyanine3 azidesulfo-Cyanine3 azide, 水溶的Cyanine3 azideCyanine5 alkyne, Cy5的类似物Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine7.5是NIR（近红外光谱）染料，是长波段的红外荧光。该叠氮类衍生物用于点击化学。可以标记多种生物分子，近红外荧光染料，是Cy7.5®改进的衍生物。标记的该荧光染料可以利用NIR组织透明度而进行体内成像实验。这种荧光素亦可用于其他荧光类应用，尤其当需要较低的荧光背景时。该叠氮染料还有溶于DMSO的溶液形式可供选择，即用于一般的点击化学的标记。或者粉剂形式自行标记使用。Cyanine7.5的桥连聚亚甲基链的结构特点会使量子产率提高20%，信号更强。

Cyanine7.5 azide

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

硫醇单一活化Cyanine3 dye。该染料用于向包含半胱氨酸残基的蛋白、多肽以及硫醇化的分子（比如含硫醇的寡核苷酸）标记Cyanine3荧光团（Cy3®的类似物）。胱氨酸应该在标记前先使用TCEP（tris-carboxyethylphosphine）或其他还原剂进行还原反应。使用Cyanine3 maleimide进行的标记反应是有选择性的，有效的。我们推荐使用水溶的Sulfo-Cyanine3 maleimide标记抗体和其他敏感蛋白。

Cyanine3 maleimide

推荐手册：

蛋白与其他硫醇分子的马来酰亚胺标记

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Perylene属于PAH（polycyclic aromatic hydrocarbon，多环芳烃）包含5个稠环。其分子的平面性造成了它的稳定性，衍生物的低溶解性以及强烈的荧光性。 Perylene拥有强烈的绿色荧光，很好的耐光性，以及量子产率的统一性。这使得这个PAH结构分子最有希望用于新的分子探针的设计，功能材料，以及分子器件。该分子包含用于点击化学的炔烃基团，可以用于像Sonogashira反应一样的偶联反应。

General properties

good in chlorinated organic solvents (DCM, chloroform), moderate in DMF, low in alcohols

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cyanine5 azide标记试剂用于点击化学，有10mM 溶解于DMSO和粉剂两种形式选择。该叠氮类染料溶于有机溶剂（如DMSO，DMF），所以标记反应需要有少量有机共溶剂的存在。该染料可用于在水和有机溶剂的混合物中标记炔烃修饰的生物分子。溶解在DMSO中的溶液形式是用于这种生物偶联的即用溶液。水溶的该染料产品：Sulfo-Cyanine5 azide。

Cyanine5是Cy5®的类似物，最广泛使用的荧光素之一，能与多种仪器相兼容。Cyanine5可以替代Alexa Fluor 647和DyLight 649。

Cyanine5 azide

Cyanine5是Cy5®的类似物，最广泛使用的荧光素之一，能与多种仪器相兼容。Cyanine5可以替代Alexa Fluor 647和DyLight 649。

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Free Cyanine3 carboxylic acid(Cy3 carboxylic acid类似物)，非活性染料，非磺化试剂，在有机溶剂中有很高的溶解度，微溶于水。该染料可作为非活性荧光团用于对照试验和校准。如果需要与胺类偶联和标记，请参考使用Cyanine3 NHS ester,或者水溶的 sulfo-Cyanine3 NHS ester。

Cy is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Pyrene是结构最简单的多环芳香族化合物（PAHs）。已知的Pyrene衍生物可以嵌入dsDNA. Pyrene具有内在荧光。当两个pyrene分子距离很近时，激发态原子就可以形成荧光光谱。因此，pyrene可以用做生物分子的探针。 Ethynylpyrene分子包含用于点击化学的三键片段，可以用于像Sonogashira反应一样的偶联反应。通过纯化可以得到纯的PAH分子。不像其他由于氧化而发棕色的商业化的产品，我们的产品是发亮绿色的。

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cyanine3.5用于点击化学，有两种形式选择：粉剂和溶解在DMSO（浓度：10mM）中的溶液形式。可以替代Cy3.5®，Alexa Fluor 594和DyLight 594。

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine3 dye azide可以用于点击化学，是Cy3® azide的类似物。Cy3®是最广泛使用的荧光素之一，因为它可以被各种不同的荧光计、成像仪和显微镜检测到。因其固有的高的消光系数，在溶解状态，肉眼也可以很容易观察到在凝胶上的Cyanine3。本产品是非磺化的，需要如DMF，DMSO或其他有机溶剂共溶剂来有效的在水中进行标记。水溶的该染料产品：Sulfo-Cyanine3 azide。该产品有两种形式可供选择：粉剂和在我们的推荐手册中提到的溶解在DMSO（浓度：10mM）中的即用型溶液形式。Cyanine3荧光素与Cy3®特性相同，类似与Alexa Fluor 546, and DyLight 549。

Cyanine3 azide

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

该蓝绿色光荧光染料可以用于FAM的FRET供体。叠氮类衍生物可以用于点击化学。

General properties

Good in organic solvents (DCM, chloroform, DMF, DMSO, MeCN, alcohols), poor in water

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

FAM azide用于点击化学的标记。FAM依然是多种应用流域最流行的荧光标记物之一。大多数荧光检测仪器从读板机到荧光显微镜都可以在FAM通道使用该染料。

鉴于点击化学的广泛应用，把这种流行的染料标记到几乎所有炔烃分子上将成为可能。

FAM azide有两种形式可供选择，粉剂和在我们的推荐标记手册中的10mM浓度溶解与DMSO的溶液形式。该产品是纯化的5-isomer。FAM可以替代Alexa Fluor 488和DyLight 488。

FAM azide, 5- isomer

鉴于点击化学的广泛应用，把这种流行的染料标记到几乎所有炔烃分子上将成为可能。

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

FAM azide, 6-isomer

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

该pyrene azide 是pyreneacetic acid的衍生物。包含三甘醇链。其他两种pyrene azides（1包括荧光团介绍详情和2）。

Pyrene azide 3

该pyrene azide 是pyreneacetic acid的衍生物。包含三甘醇链。其他两种pyrene azides（1包括荧光团介绍详情和2）。

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

PEP (phenylethynylpyrene) azide pyrene衍生物有更长的激发和发射波长 Perylene azide另一个多环芳烃绿色荧光染料，也是荧光共振能量转移pyrene受体

General properties

soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

good solubility in chloroform, dichloromethane, moderate solubility in DMSO, DMF, acetonitrile

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Pyrene属于多环芳烃，拥有较强短波荧光。不像其他的荧光染料，多环芳烃类属于对微环境有较强灵敏度的荧光探针。因此，它的荧光性在极性和非极性环境中是不同的。其他的影响也可以被观察到。当两个pyrenes靠的很近时，他们便表现出激发态。激发态形式很容易被观察到，使用荧光波谱图可以被定量估测。 Pyrene azide可以比较容易的应用于pyrene点击化学，对难以进行炔烃标记的分子进行标记。它也可以将分子引入难以标记pyrene的探针。这种叠氮染料含有亲水三甘醇链以缓解其固有的疏水性并促进在水中对生物分子的标记。另两种点击化学用pyrene azides（2和3）。

Pyrene azide 1

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

PEP (phenylethynylpyrene) azide pyrene衍生物有更长的激发和发射波长

Perylene azide另一个多环芳烃绿色荧光染料，也是荧光共振能量转移pyrene受体

General properties

soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Cyanine3.5 NHS ester（Cy3.5® NHS ester的类似物）是标记多肽、蛋白的氨基基团和寡核苷酸的活性染料。可以替代Cy3.5®, Alexa Fluor 594, DyLight 594。

推荐手册

氨基分子的NHS酯标记

Cy® is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Perylene是一种高亮度的且对公极其稳定的多环芳烃（PAH）类荧光染料，其量子产率接近理想化。因其很短的荧光期，这种探针没有激发态。因其较低的溶解度和很少量的活性衍生物，Perylene标记生物分子通常比较困难且不太容易实现。然而，通过点击化学使用这种perylene azide来进行标记就变得和其他染料一样非常容易。使用这种多环芳烃染料标记多肽、DNA、蛋白等从未如此轻松。为了提高其亲水性和标记成功率，这种PAH azide包含亲水三甘醇链。

Perylene azide

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

Pyrene azide 另一个多环芳烃蓝色荧光染料 Phenylethynylpyrene (PEP) azide

General properties

good in dichloromethane and chloroform, moderate in DMSO, DMF, and acetonitrile

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

胺类活性染料，近红外荧光染料，Cy7®的改进的类似物。

近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域，高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验，如活体研究及药物开发相关实验。

Cy7的特殊结构加固了中心聚亚甲基（polymethyne）链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%，因此它的荧光强度会更大。该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。

推荐手册

氨基分子的NHS酯标记

Cy® is a trademark of GE Healthcare.

General properties

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Phenylethynylpyrene（PEP）荧光素属于多环芳烃类标记物，有较高灵敏度，是对微环境进行标记的试剂。类似于Pyrene，PEP dye很容易形成激发物。然而，PEP荧光多为红移。 PEP可以用作微环境探针，基于激发态标记实验。该试剂包含三甘醇链可以促进非极性PEP dye在有机溶剂-水相混合标记反应中的溶解。此叠氮类染料可以很容易的用于点击化学中对PEP标记探针引入炔烃分子。

PEP azide

推荐手册：

寡核苷酸与DNA的点击化学标记

相关产品：

Pyrene azide 另一个多环芳烃蓝色荧光染料 Perylene azide 另一个多环芳烃红色荧光染料

General properties

soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

ROX(rhodamine X aka R101)是一种高荧光强度的、耐光的罗丹明染料，在多领域有广泛的应用。ROX标记的寡核苷酸探针常用于qPCR和有ROX通道的qPCR仪器。该活性染料用于标记多肽、蛋白、氨基寡核苷酸的氨基基团。Pure single isomer.ROX NHS ester可以替代Alexa Fluor 568。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

TAMRA (TMR, tetramethylrhodamine) azide，有固体和10mM溶于DMSO两种形式。用于点击化学的标记试剂。是纯化的5-isomer。 TAMRA通常用于FAM荧光团的荧光共振能量转移（FRET）受体。可以替代Alexa Fluor 555, DyLight 549。

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

BDP FL NHS ester是一种在488nm通道的染料，是一种类似于BODIPY FL NHS ester的分子，还是荧光素的替代剂。用于标记蛋白和多肽的氨基活性染料。虽然这种荧光染料的吸收和发射光谱比FAM的激发和发射通道范围小，处在其范围内，但BDP FL NHS ester有着更好的耐光性，在亮光下表现突出。但是在单色光分光器上，如果能调整到染料的最大发射波长处，将检测到更大信号亮度。这种染料是中性的，小分子量，在结合物中也能保持高的量子产率。该染料能够很好的替代fluorescein (FAM), BODIPY-FL, Alexa Fluor 488, DyLight 488, Cy2及其他在488nm纳米处激发的荧光染料。

BODIPY is a trademark of Life Technologies.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

水溶近红外染料sulfo-Cyanine7，是胺类活性琥珀酸亚胺酯。

Sulfo-Cyanine7是Cy7®荧光团的的改良衍生物，量子产率提高20%，并具有更好的耐光性。该染料尤其适用于NIR成像应用。

近红外荧光染料利用了生物组织在特定的波长范围里的透明度。这种方法是无损伤性的，并允许在活体内对多种已标记的生物分子的分布进行跟踪。

Sulfo-Cyanine7 NHS ester可以很轻松的标记生物分子，如：蛋白质。标记的分子可以用于后续多种研究及药物开发相关的实验。

这种染料有很高的水溶解度，尤其适用于痕量蛋白质和易变性蛋白质的标记。需有机溶剂才能溶解的非磺化的Cyanine7 NHS ester。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

用于点击化学的水溶Cyanine5 dye azide。高亮度耐光的红色染料。在水中的溶解度较高。由于其高溶解度和亲水性，该染料在单纯的水环境中即可对生物分子进行标记。 Sulfo-Cyanine5是Cy5 的类似物，是一种非常流行的荧光染料，能与很多荧光设备相兼容，比如成像仪，读板机，显微镜等。该试剂是1:1混合的crystallosolvate和methanol。但不会对荧光团性能和应用有任何影响。

Sulfo-Cyanine5 azide

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

水溶，氨基活性sulfo-Cyanine3 NHS ester。不需有机溶剂，在水溶液中即可有效的对蛋白和多肽进行标记。该产品是低溶解度蛋白和易变性蛋白的理想选择。这是一种磺化的、亲水的、水溶的荧光染料。没有磺化的Cyanine3 NHS ester亦可供选择。该产品是Cy3® NHS ester的衍生物。在所有应用中Sulfo-Cyanine3 NHS ester都可以替代Cy3®，Alexa Fluor 546和DyLight 549。

General properties

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Sulfo-Cyanine3 azide是用于点击化学的水溶叠氮染料。其激发和发射波长与Cy 荧光团相同。由于这种染料的高亮度，耐光性，再加上在水中高溶解度的特点，该染料成为点击化学中水相标记各种分子的理想选择。在水相中不需有机共溶剂即可进行点击反应（Click reaction）。该染料是为标记敏感的分子如蛋白甚至完整的生物体而设计。该试剂是1:1混合的crystallosolvate和methanol。但不会对荧光团性能和应用有任何影响。

Sulfo-Cyanine3 azide

推荐手册：

寡核苷酸与DNA的点击化学标记

Cy is a trademark of GE Healthcare.

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

ROX (rhodamine-X) azide，有固体和10mM浓度溶于DMSO的溶液两种形式选择。用于点击化学的标记试剂。ROX属于发射红光的罗丹明（rhodamine）荧光染料具有高亮度和荧光量子产率。纯化的5-isomer。可以替代Alexa Fluor 568。

ROX azide, 5- isomer

推荐手册：

寡核苷酸与DNA的点击化学标记

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

R110 (rhodamine 110) azide，有固体和10mM浓度溶于DMSO的溶液两种形式选择。用于点击化学的标记试剂。纯化的6-isomer。与荧光素的光物理性质类似，但更耐光。可以替代Alexa Fluor 488，DyLight 488和FAM (fluorescein)。

R110 azide, 6- isomer

推荐手册：

寡核苷酸与DNA的点击化学标记

General properties

Storage: 24 months after receival at -20 C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

水溶Cyanine 5琥珀酰亚胺酯（succinimidyl ester（SE）），类似于Cy5® NHS ester，不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。

Sulfo-Cyanine 5是Cy5®的衍生物，与很多仪器如读板机（plate readers），显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。

该染料是高度亲水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。

可以在所有的应用中替代Cy5®，Alexa Fluor 647和DyLight 649。

上海金畔生物科技有限公司

Cy5.5 NHS是近红外胺类活性染料。

CY5.5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。CY5.5属于是近红外荧光染料，当背景荧光有干扰时适宜用它来做标记以进行荧光分析。它也可以用于活体成像实验。该染料可以替代Alexa Fluor 680和DyLight 680。

上海金畔生物科技有限公司

文章号：6-6

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