Biotin azide is a derivative of biotin (vitamin H) covalently bound with azide group. This reagent allows labeling of various alkynylated molecules (such as DNA, oligonucleotides, and proteins) with biotin. Biotin binding to avidin or streptavidin can be used in downstream affinity applications for the isolation of biotinylated molecules or their binding with streptavidin conjugates.

This structure of biotin azide features long hydrophilic linker which separates biotin residue from the target molecule for efficient binding with streptavidin. Its linker (PEG) also enhances aquous solubility to facilitate conjugation.

The azide can be conjugated with various molecules using our recommended protocol.

Recommended protocol

Click-Chemistry Labeling of Oligonucleotides and DNA

General properties