产品说明
RNA/DNA/ProteinPurification96-WellPlusKitThis96-wellkitprovidesarapidmethodforthehighthroughputisolationandpurificationoftotalRNA,DNA andproteinssequentiallyfromasinglesampleofculturedanimalcells,smalltissuesamples,blood,bacteria,oryeast. Thekitemploystwoplates:1)forDNApurificationand2)forRNApurificationutilizingsiliconcarbideresin(superiorforthebindingofallRNAsizesincludingmiRNA). Pleaseseetheprotocol schematic below.TheproteinsarealsopurifiedonthesecondplateafterRNAelution. Theproteinsareelutedinbufferandarereadyfordownstreamapplicationswithoutanyfurthercleanuprequired.Theproteinscanbequantifieddirectly,usedinwesternblots,ELISAormassspectrometry. Norgen"sRNA/DNA/ProteinPurification96-WellPlus Kitisespeciallyusefulforresearcherswhoareisolatingmacromoleculesfromprecious,difficulttoobtainorsmallsamplessuchasstemcells,CTC,biopsymaterialsorsinglefocifromcellcultures,asiteliminatestheneedtofractionatethesample.FurThermore,analysiswillbemorereliablesincetheRNA, DNA andproteinsarederivedfromthesamesample,therebyeliminatinginconsistentresults.Thepurifiedmacromoleculesareofthehighestpurityandcanbeusedinanumberofdifferentdownstreamapplications.Figure1.HighThroughputIsolationofHighQualityRNAwithCompleteSizeRangewithouttheUseofPhenol.Norgen"sRNA/DNA/ProteinPurification96-WellPlusKitallowsfortheconsistentisolationofhighqualityRNA,withcompletesizerangefromtheverylargeRNAdowntosmallRNAwithouttheuseofphenol.HeLaRNAandhamsterliverRNAwasisolatedusingNorgen"sRNA/DNA/ProteinPurification96-WellPlusKitinreplicates.(A)TheisolatedRNAwasresolvedona1.2%formaldehydeagarosegel.Allreplicatesshowedbothhighyieldandhighquality.Inaddition,allreplicatesshowedeffectiverecoveryofallsizesofRNAincludingthesmallRNA(arrow).(B)TheisolatedRNAwasresolvedonanAgilentRNANano6000Chip.AllRNAachievedhighRNAIntegrityNumber(RIN).TheRNAisolatedbyNorgen"sRNA/DNA/ProteinPurification96-WellPlusKitshowedgoodrecoveryofbothsmallRNA(miR-21,(C))andlargeRNA(beta-Actin,(D)).]">RNA/DNA/ProteinPurification96-WellPlusKitFigure1Figure2RecoveryofIntact,HighQualityGenomicDNAfromHeLacellsandHamsterLiver.PanelAisa1%agarosegelshowingthegDNAisolatedfromthesameHeLacellorhamsterliversamplesusingNorgen"sRNA/DNA/ProteinPurification96-WellPlusKitwithNorgensHighRanger1kbDNALadderandthesamplelanescontain10µLofeachofthe100µLelutions.Thegelshowedhighquality,andintactgenomicDNA.PanelBistheresultofqPCRamplificationof25ngofelutedgenomicDNAusing5SrRNA-specificprimers.GenomicDNAisolatedusingNorgen"sRNA/DNA/ProteinPurification96-WellPlusKitisofhighqualityandiscompatIBLetosensitivedownstreamapplications.]">RNA/DNA/ProteinPurification96-WellPlusKitFigure2Figure3.ConsistentIsolationandPurificationofTotalProteinfromthesamesampleusedforRNAandDNAIsolation.Norgen"sRNA/DNA/ProteinPurification96-WellPlusKitallowssequentialisolationofRNA,DNAandproteinfromthesamesamplewithoutsplitting.TheflowthroughsamplesfromtheRNA/DNAisolationinFigure1andFigure2werefurtherpurifiedusingNorgen"sRNA/DNA/ProteinPurification96-WellPlusKit.Tenmicrolitersofthe100µLproteinelutionswereloadedona10%SDS-PAGEgel.Theproteinsareconsistentinyield,intactandofthehighestquality,andcanbeusedinanumberofdifferentdownstreamapplications.]">RNA/DNA/ProteinPurification96-WellPlusKitFigure3
ATCC关于
ATCC是全球领先的生物材料资源和标准组织，其任务集中于标准参考微生物，细胞系和其他材料的获取，认证，生产，保存，开发和分发。ATCC在保留传统收集材料的同时，还开发了高质量的产品，标准和服务，以支持科学研究和突破性发展，从而改善全球人口的健康状况。
历史
ATCC成立于1925年，当时的一个科学家委员会认识到需要集中收集可服务于全世界科学家的微生物。最初的几年是在芝加哥的麦考密克研究所度过的，直到该组织于1937年移至华盛顿特区的乔治敦大学。随着生物科学领域的研究不断扩展，ATCC开始多元化经营，随着藏品的增长，ATCC占据了一系列地点，每个都提供更多的存储空间。ATCC于1998年搬到了现在的位置。
设备
ATCC总部和生物生产设施位于弗吉尼亚州的马纳萨斯，靠近国家首都和美国国立卫生研究院。这栋126,000平方英尺的建筑物由现场安全人员持续监控，并由现场发电机提供电力支持。该设施的储存库部分占地18,000平方英尺，包含200个用于存储生物材料的冰柜，包括气相液氮冰柜，机械冰柜和冷藏室。仓库面积补充了35,000平方英尺的实验室空间。
新品排行榜
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ATCC(美国菌种保藏中心)/TNTU/024890-25g
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ATCC(美国菌种保藏中心)/N-Butanol, ACS, 9...
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ATCC/Pyridine-4-boronic acid hyd...
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ATCC(美国菌种保藏中心)/Anisole/M03556-18...
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ATCC(美国菌种保藏中心)/Anisole/M03556-5g
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ATCC/Pyridine-4-boronic acid hyd...
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ATCC(美国菌种保藏中心)/Copper(II) tetraf...
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ATCC/3,3,3-Trifluoropropylmercap...
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ATCC/Trifluoromethanesulfonic ac...
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ATCC/4,5-Dicyanoimidazole/013519...
