Highest biotin-binding streptavidin magnetic beads on the market
Over the past 10 years, TriLinks streptavidin-coupled NanoLink™ Beads have been widely used in types of organizations and areregularlycited in papersfor diverse manual and automated applications.
NanoLink™ Streptavidin Magnetic Beads have the highest biotin-binding capacity on the market. Higher binding translates into reduced bead mass required to immobilize a biotinylated sample and lower background noise from nonspecific binding, resulting in better signals and lower net costs.
Download White Paper: How to Maximize the Capture of Biotinylated Targets with Streptavidin Magnetic Beads
NanoLink™ Outperforms Competitors on Free-Biotin Binding Capacity
![](\"/cart/ProdImages/thumbs/graph_medium.jpg\")
![](\"/cart/ProdImages/thumbs/nanolink_CG_QP_large_medium.jpg\")
The secret is in the crosslinkingNanoLink™ Streptavidin Magnetic Beads are 1.0micron-sized, super-paramagnetic, hydrophobic, and polyme–encapsulated (no exposed iron), with a fast (<2 min) magnetic response time. They are stable in colloidal form and in detergents. The key to high biotin binding is in the unique covalently crosslinked streptavidin, based onChromalink™ technology. The high surface area, when combined with our efficient linking chemistry, produces a consistent, ultra-high, biotin-binding bead. The high surface area and lower nonspecific binding makes NanoLink™ Steptavidin Magnetic Beads ideal for immobilizations and Co-IP applications.
NanoLink™ Binding Capacity vs. Competition
Ligand | NanoLink (1.0μm) Binding | Competitors 1 μm Bead Binding |
Free biotin | >14 nmol/mg | >1,300 pmol/mg |
Biotinylated oligo (23-mer) | >2.5 nmol/mg | NA |
Biotinylated IgG (3 biotins per IgG) | >1.7 nmol/mg (250 μg/mg) | 0.12 nmol/mg (20 μg/mg) |
Advantages
- Highest Biotin Binding – Supported by unique streptavidin crosslinking
- Fast (<2 min) Response Time – Saves time and accommodates viscous samples
- Versatile – NanoLink™ beads are ideal for immobilizations and Co-IP applications
Applications
- Capture, immobilization, and separation of biotin-labeled biomolecules such as genomic DNA, RNA, PCR products, oligonucleotides, peptides, or antibodies. We recommend biotinylation with the ChromaLink™ Biotin Labeling Kit (Cat. No. B-9007-105K).
- High-throughput robotic applications where high biotin-binding loads must be removed or immobilized without the presence of an iron leachate.
- NanoLink™ Streptavidin Magnetic Beads are ideally suited for generating single-stranded PCR templates that can dramatically increase hybridization efficiency to complementary probes by removal of the unbiotinylated, competing PCR strand.
Additional Information
- Product Insert and Protocol
- Product Data Sheet
- MSDS
Citations
Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.
