产品名称 : Endothelin-1 Antibody;Endothelin 1 抗体
产品类别 : 一抗
品 牌 : ABBIOTEC
货 号 : 250633
规 格 : 0.1 mg
克隆类型 : Rabbit Polyclonal Antibody
亚 型 : Rabbit Ig
免 疫 原 : KLH-conjugated synthetic peptide corresponding to human proendothelin-1.
产品描述 :
Endothelins are endothelium-derived vasoconstrictor peptides. Endothelin-1 (ET-1) is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Endothelin-1 is expressed as a 212-aa precursor (preproendothelin-1) that is sequentially cleaved into 2 chains: Endothelin-1 (21 aa) and Big Endothelin-1 (38 aa). This antibody recognizes an epitope in the N-term prodomain of Endothelin-1.
适用物种 :
H, M, R, Other, B, P
应用范围 :
E, WB, IHC
应用说明 :
Product Cat. No. 250633 recognizes an epitope in the N-term prodomain of Endothelin-1. E: 1:500-1:1,000; WB: 1:200-1:500; IHC: 1:200-1:500
别 名 :
Endothelin-1; preproendothelin-1; PPET1; ET-1; EDN1
形 态 :
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 25% glycerol, 5 mg/ml BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.
保存条件 :
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
相关文献 :
[1] Kim J et al. 2013. PLoS ONE. 8(9):e74458. PMID# 24040253. [2] Fradet A et al. 2013. PLoS ONE.8(9):e75092. PMID# 24069383. [3] DeNiro M et al. 2013. PLoS ONE. 8(3): :e59021. PMID# 23533599. [4] Jo S et al. 2011. Elsevier. Neuroscience Letters. 510(1):25-30. PMID# 21742016. [5] Ko G-J. et al. 2010. Am. J. Physiol. Renal Physiol. 298(6):F1472-F1483. PMID# 20181666.
实验图片 :
Fig.1 Endothelin-1 staining in mouse vascular endothelium. Paraffin-embedded mouse vascular endothelium is stained with Endothelin-1 Antibody (Cat. No. 250633) used at 1:100 dilution.Fig.2 250633: The Endothelin-1 Antibody (Cat. No: 250633) is used at 1:200 in Western blot with rat brain lysate to detect Endothelin-1.Fig.3 Neurons and endothelial cells from rat brain sections are stained in red with Endothelin-1 Antibody (Cat. No. 250633) used at 1:500 dilution (data from Kim JE et al. 2013).
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