期刊名称： Proceedings of the National Academy of Sciences of the United States of America We have developed a method for isolation of random peptides that inhibit intracellular processes in bacteria. A library of random peptides expressed as fusions to Escherichia coli thioredoxin (aptamers) were expressed under the tight control of the arabinose-inducible P(BAD) promoter. A selection was applied to the library to isolate aptamers that interfered with the activity of thymidylate synthase (ThyA) in vivo. Expression of an aptamer isolated by this method resulted in a ThyA(-) phenotype that was suppressed by simultaneous overexpression of ThyA. Two-hybrid analysis showed that this aptamer is likely to interact with ThyA in vivo. The library also was screened for aptamers that inhibited growth of bacteria expressing them, and five such aptamers were characterized. Four aptamers were bacteriostatic when expressed, whereas one showed a bactericidal effect. Introduction of translational stop codons into various aptamers blocked their activity, suggesting that their biological effects were likely to be due to protein aptamer rather than RNA. Combinatorial aptamers provide a new genetic and biochemical tool for identifying targets for antibacterial drug development. 人畜共患疾病

以农业部人畜共患传染病名录为权威指引，将每一个病种进行基本面介绍，充分关注社会民生关注的焦点热点，加强公众对该方面知识的了解、学习和宣传实现跨界型农科与医科的结合，形成优势互补。

中国农业科学院农业信息研究所 2017中国工程科技知识中心 2017中国工程科技知识中心 ICP备案号：京ICP备14021735号-2