由于CST严格的内部交叉验证标准(确保特异性、灵敏度、批次间一致性),所有经IHC-P验证通过的CST抗体都可以应用在利用TSA技术的mIHC中。
多重荧光免疫组化(mIHC)是化学显色免疫组化和荧光标记抗体之外的另一种信号检测方式,其应用酪胺信号放大技术(TSA®), 可在同一石蜡包埋组织切片中检测多达7个以上靶标。
IHC应用配套试剂
mIHC 商业化抗体试剂盒CST应用技术指南及手册:Fluorescent multiplex immunohistochemistry with tyramide signal amplification.Characterize immune checkpoint proteins and T cell exhaustion using multiplex IHC.Characterize the immunosuppressive tumor microenvironment using multiplex IHC.Visualize immune in infiltrate & checkpoint expression in murine syngeneic models.肿瘤免疫研究手册CST学术海报:Zeillo, J., et al., Highly multiplexed IHC assays to examine immune checkpoints and biomarkers for immunotherapy.
Ziello, J., et al., Co-expression and frequency of immune checkpoint proteins in the tumor microenvironment analyzed via multiplex IHC.
Zeillo, J., et al., Multiplex IHC Detection of Immune Checkpoint Receptors in the Tumor Microenvironment.Ziello, J., et al., Characterization of immune infiltrate and checkpoint protein expression patterns in murine syngeneic tumors via multiplex IHC. Klein, S., et al., PD-1 blockade activates conventional CD4+ T cells and the innate immune response during glioblastoma eradication.CST通路挂图:Tumor immunology: examining the microenvironmentTumor ImmunologyCST已优化mIHC抗体组合:为了让大家可以轻松开展mIHC实验,CST的科学家进行了无数的实验测试,已经优化了一系列mIHC抗体组合,欢迎您填写右侧/下侧表单进行下载。若以上已优化Panel信息无法完全满足您的mIHC实验需求,或您还需要其他关于mIHC的技术支持,请您向我们反馈:点击提交。 *注:Alexa Fluor、DyLight是Thermo Fisher Scientific公司注册商标;Cy 是GE Healthcare公司注册商标;TSA是PerkinElmer公司注册商标。- End -
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