Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr450 of human DENND3.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20°C is recommended. Stable at –20°C for at least 1 year.
General References
McPherson PS, Xu J (2015). DENND3: a signaling/trafficking interface in autophagy. Cell Cycle. 17: 2717-8.
Matsui T, Itoh T, Fukuda M (2011). Small GTPase Rab12 Regulates Constitutive Degradation of Transferrin Receptor. Traffic. 10: 1432-43.
McPherson PS, Dokainish H, Marat AL (2011). DENN Domain Proteins: Regulators of Rab GTPases. J Biol Chem. 16: 13791-800.
