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GammaIrradiationofPMEFs-资讯wiki版
Thawing Cells-Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC.-Add the 1ml of cell containing media to 9ml of PMEF media in a 15ml falcon tube and spin on setting one for five minutes. This procedure removes the DMSO.-Aspirate media off the cell pellet and resuspend gently in 1ml of media using a 1ml pipette.-Add the......阅读全文


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