Restriction Digests of High Molecular Weight Yeast DNA - 酵母...
To perform restriction digests of YACs for mapping using rare cutting enzymes or more conventional restriction endonuclease digestion. Time required: 2-4 hours Special Reagents: dependent upon enzymes desiredProcedure: Using wide-bore pipette, aliquot 100 ng of liquid DNA into eppendorf tube. Add 2.5 祃 10X restriction buffer, 1-2 ul RNase (5 mg/ml stock), 250-500 ng BSA, then dH2O up to 25 祃 volume. Incubate at appropriate temperature for 2-4 hours. Stop the reaction by adding 3 祃 of 10X glycerol stop mix; mix in tube gently, then microfuge for 15 seconds (rather than pipette up and down, which may shear the DNA).联系人：蚂蚁淘编辑部Email：service@bioon.com电话：021-54485309传真：021-54485087
