Description: SbmA, pAb

This polyclonal antibody recognizes the inner membrane protein SbmA of Gram-negative bacteria. The SbmA protein is a homodimeric secondary transporter protein, that is involved in the transport of microcins B17 and J25, glycopeptide antibiotic bleomycin, proline-rich antimicrobial peptides of prokaryotic and eukaryotic origin, antisense peptide phosphodiamidate morpholino oligomers and peptide nucleic acids (PNA) into the E.coli cytoplasm. This protein is found in a large number of Gram-negative and also Gram-positive microorganisms, including plant and animal pathogens. The monomeric SbmA protein contains 406 amino acids (about 50 kDa) and has 8 transmembrane regions. The homodimeric complex present in the membrane closely resembles the membrane-spanning region of the ATP-binding cassette transporter family. Peptide transport mediated by SmbA is driven by a electrochemical gradient. SbmA-mediated internalization of peptide substrates suggest that the transport of an unknown substrate(s) represents the function of this protein.

Specifications:

Catalog number Product type Quantity Formulation Application Application Notes Use Immunogen Isotype Species Positive Control References Storage and stability Precautions Disease

HP6001

Polyclonal antibodies

100 µg

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Immuno precipitation, Western blot

W: The band size to be expected is ~37 kDa under reducing conditions.

For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Recombinant SbmA

Rabbit Ig

N/A

Recombinant SbmA or E.coli lysate

1. Ghosal, A et al; Role of SbmA in the uptake of peptide nucleic acid (PNA)-peptide conjugates in E. coli. ACS Chem Biol 2013, 8: 360 2. Corbalan, N et al; Functional and structural study of the dimeric inner membrane protein SbmA. J Bacteriol 2013, 195: 5352 3. Runti, G et al; Functional characterization of SbmA, a bacterial inner membrane transporter required for importing the antimicrobial peptide Bac7(1-35). J Bacteriol 2013, 195: 5343

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Infectious diseases