TOP2A-Specific Polyclonal antibody TOP2A-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA Validation Data GalleryWB analysis of HepG2 using 20233-1-APWB result of TOP2A-Specific antibody (20233-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A-Specific transfected HepG2 cells.View All Images (10)×WB result of TOP2A-Specific antibody (20233-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A-Specific transfected HepG2 cells.WB analysis of HeLa using 20233-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.View All Images (10)×HeLa cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.IHC staining of human spleen using 20233-1-APImmunohistochemical analysis of paraffin-embedded human spleen using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens).View All Images (10)×Immunohistochemical analysis of paraffin-embedded human spleen using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens).IF Staining of HepG2 using 20233-1-APImmunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).View All Images (10)×Immunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).IP experiment of HeLa using 20233-1-APIP Result of anti-TOP2A-Specific (IP:20233-1-AP, 4ug; Detection:20233-1-AP 1:500) with HeLa cells lysate 2000ug.View All Images (10)×IP Result of anti-TOP2A-Specific (IP:20233-1-AP, 4ug; Detection:20233-1-AP 1:500) with HeLa cells lysate 2000ug.-/ - Cat No. 20233-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHeLa cells, L02 cells, A431 cells, HepG2 cellsPositive IP detected inHeLa cellsPositive IHC detected inhuman spleen tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHepG2 cells Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:3000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBImmunohistochemistry (IHC)IHC : 1:20-1:200Immunofluorescence (IF)IF : 1:10-1:100Sample-dependent, check data in validation data gallery 20233-1-AP targets TOP2A-Specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples. Calculated molecular weight 174 kDaObserved molecular weight 170-177 kDaGenBank accession numberNM_001067 Gene symbol TOP2A Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationTOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation. ProtocolsProduct Specific ProtocolsWB protocol for TOP2A-Specific antibody 20233-1-APDownload protocolIHC protocol for TOP2A-Specific antibody 20233-1-APDownload protocolIF protocol for TOP2A-Specific antibody 20233-1-APDownload protocolIP protocol for TOP2A-Specific antibody 20233-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (11)KD/KO (2)WB (8)IF (2)SpeciesApplicationTitlehumanWBBiomed Res IntPMID: 31662984Authors - Yi FangView ArticlehumanWBCancer Treat Res CommunEtoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.Authors - Carlo CattriniView ArticlehumanWBMol Med RepHigh glucose promotes gastric cancer chemoresistance in vivo and in vitro.Authors - Wei ZhaoView ArticleOncol RepAnticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.Authors - Lei GaoView ArticlehumanWBJ CancerIdentifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis.Authors - Yaoyi XiongKD ValidatedView ArticlehumanWBInt J Mol SciPMID: 32599901Authors - Martina GodelView Article See more citations WB FiguresWB analysis of A431 using 20233-1-APA431 cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.WB analysis of HeLa using 20233-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.WB analysis of HepG2 using 20233-1-APWB result of TOP2A-Specific antibody (20233-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A-Specific transfected HepG2 cells.WB analysis of L02 using 20233-1-APL02 cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.WB analysis of L02 using 20233-1-APL02 cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.WB analysis of L02 using 20233-1-APL02 cells were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC FiguresIHC staining of human spleen using 20233-1-APImmunohistochemical analysis of paraffin-embedded human spleen using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:100 (under 10x lens).IHC staining of human spleen using 20233-1-APImmunohistochemical analysis of paraffin-embedded human spleen using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens).IP FiguresIP experiment of HeLa using 20233-1-APIP Result of anti-TOP2A-Specific (IP:20233-1-AP, 4ug; Detection:20233-1-AP 1:500) with HeLa cells lysate 2000ug.IF FiguresIF Staining of HepG2 using 20233-1-APImmunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). \"TOP2A-Specific antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare. WB : 1:500-1:3000IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC : 1:20-1:200IF : 1:10-1:100