染色质免疫沉淀 (ChIP) 是一种流行且强大的技术，用于确定 DNA - 蛋白质相互作用。然而，ChIP 对于初学者来说可能相当令人畏惧，因为它是一项漫长而复杂的技术，有许多选项和决定，开始容易但完成困难！
在本次演讲中，我们将讨论 3 ChIP 的各个阶段，以及每个阶段的实用技巧和策略，可增加您了解 ChIP 结果的机会，使 ChIP 像 1、2、3 一样简单！染色质免疫沉淀 (ChIP) 研讨会 - 由伦敦大学学院的 Toryn Poolman 博士主持。
ELISA 实验理论上很简单，但实际上很难产生可靠且准确的结果。加入我们的互动研讨会，了解 ELISA 最佳实践和优化技巧，确保您从检测中获得最佳结果。我们与研发科学家密切合作，利用 20 年 ELISA 制造经验为您提供最新的专家建议。本研讨会适合所有人，从初学者到 ELISA 专家。
正在寻找您的下一个博士后或研究员，或正在考虑从学术界转向工业界？制作一份优秀的简历是获得重要面试机会的第一步。与学术界和工业界的专业人士一起获取宝贵的建议，帮助您在职业生涯中迈出下一步。包括深入的问答和出席证书。
其他与专家会面：Steve Chan 博士 - 乳腺癌的表观遗传调控与专家会面：Steve Chan 博士 - 乳腺癌的表观遗传调控
使用公正的 ChIP-Rx 分析发现 CTR9 在乳腺癌中的新作用乳腺癌中 PRC2 亚型转换的表观遗传调控
Proteintech 很高兴接待来自威斯康星大学麦迪逊分校的 Steve Chan 博士，他向我们介绍了他们最近的论文\"转录延伸因子 CTR9 划分 PRC2-通过改变 PRC2 亚型平衡来介导 H3K27me3 结构域”，发表在《核酸研究》杂志上。Chan 及其同事在 Wei Xu 教授的监督下，对组蛋白修饰进行了定量测量以及无偏 ChIP-Rx 分析，以表明 CTR9 控制乳腺癌细胞中 H3K27me3（一种组蛋白修饰剂）的水平和基因组分布。 CTR9 耗尽深刻影响 PRC2 亚型转换并产生漏洞，导致乳腺癌细胞对 PRC2 抑制剂过敏。他们的研究揭示了一种机制，为使用 EZH2 抑制剂治疗靶向 CTR9 低乳腺肿瘤提供了基础。
OtherLab 生活平衡第 2 部分：MentalLab 生活平衡第 2 部分：心理
通过观看我们的网络研讨会，了解如何更好地改善您的实验室/生活平衡。在我们的第二部分中，专家们讨论有关心理健康的话题。观看下面的视频，了解保持平衡生活的提示和技巧。
通过观看我们的网络研讨会，了解如何更好地改善您的实验室/生活平衡。在我们的第一部分中，专家们讨论有关身体健康的话题。观看下面的视频，了解保持平衡生活的提示和技巧。
其他生命科学家的 R 编程简介生命科学家的 R 编程简介
R 广泛用于生物数据集的分析。然而，R 很复杂，需要经验才能有效使用。来自伦敦大学学院的 Toryn Poolman 博士将带我们了解 R 的一些基本功能，这对所有生命科学家都有用。之前使用 R 的一些经验很有用，但不是必需的。
Toryn 将使用 R 带领我们了解他自己研究中的真实数据集。本研讨会将涵盖：
现实数据集的统计分析创建多面板图形向图形添加统计数据使用 R markdown 注释图形可以在此处找到本研讨会中使用的数据集示例的链接：https://github.com/toryn13/Rexamples
Phospho-TDP43 (Ser409/410) Polyclonal antibody Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB,ELISA Synonyms phospho TDP43, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TARDBP, TDP43 Validation Data GalleryWB analysis using 22309-1-APNon-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.Enlarge Image×Non-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. -/ - Cat No. 22309-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inCalyculin A treated HeLa cells Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included. Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:2000Sample-dependent, check data in validation data gallery 22309-1-AP targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples. Calculated molecular weight 43 kDaObserved molecular weight 40-50 kDa, 25-35 kDaGenBank accession numberNM_007375 Gene symbol TARDBP Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationTransactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining. ProtocolsProduct Specific ProtocolsWB protocol for Phospho-TDP43 (Ser409/410) antibody 22309-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (45)WB (16)IHC (21)IF (15)SpeciesApplicationTitlehumanWB,IFJ Biol ChemAggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.Authors - Youjun ChenView ArticlehumanWB,IHC,IFLab InvestAssociation between TDP-43 and mitochondria in inclusion body myositis.Authors - Mikayla L HuntleyView ArticleJ Alzheimers DisPhospho-Tau Changes in the Human CA1 During Alzheimer\'s Disease Progression.Authors - Mamen Regalado-ReyesView ArticleFuture Sci OAPlatelet phosphorylated TDP-43: an exploratory study for a peripheral surrogate biomarker development for Alzheimer\'s disease.Authors - Rodger WilhiteView ArticlehumanIHC,IFActa Neuropathol CommunPhosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis.Authors - Matthew D CykowskiView ArticlehumanIHCActa Neuropathol CommunFragile X-associated tremor ataxia syndrome with co-occurrent progressive supranuclear palsy-like neuropathology.Authors - Amanda N SacinoView Article See more citations ReviewsAdd a reviewThe reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.FHAzita (Verified Customer) (03-10-2020)Immunofluorescent analysis of (4% PFA fixed) NSC-34 cells, using p- (109/110) TDP-43 antibody at 1/500 dilution and Alexa Flour conjugated Donkey anti Rabbit IgG (H+L) (Red). Bisbenzimide (Blue) labels cell nuclei.Applications: Immunofluorescence,Primary Antibody Dilution: 1/500Cell Tissue Type: NSC-34 motor neuron like cell linePhospho-TDP43 (Ser409/410) Antibody Immunofluorescence, validation (1/500 dilution) in NSC-34 motor neuron like cell line (Cat no:22309-1-AP)FHLaura (Verified Customer) (01-10-2020)Works well at this concentration on HEK293T lysate.Applications: Western Blot,Primary Antibody Dilution: 1:500FHOwen (Verified Customer) (10-30-2019)This antibody was used to image TDP43 in tissue and cell samples. The antibody was useful to image such a difficult target.Applications: Immunofluorescence,Primary Antibody Dilution: 1:50Cell Tissue Type: Human cortical tissue / motor neurons WB FiguresWB analysis using 22309-1-APNon-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. \"Phospho-TDP43 (Ser409/410) antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare.
