了解非结构蛋白 (NSP) 的作用及其对 SARS-CoV-2 感染的长期严重影响的影响
自 COVID-19 大流行爆发以来，对冠状病毒的研究；特别是 SARS-CoV-2，呈指数级增长。病毒刺突蛋白以及 ACE2 和 TRPMSS2 等靶标的研究一直是最热门的研究主题。了解非结构蛋白 (NSP) 的作用及其对 SARS-CoV-2 感染的长期严重影响的影响是了解严重疾病症状的重要一步。
NSP 有助于 SARS -CoV-2病毒通过内质网（ER）劫持和免疫逃避建立复制和转录复合体（RTC）。 RTC 和膜重排会导致细胞器损伤，例如 ER 应激、自噬机制变化、高尔基体断裂、和溶酶体膜的改变。这被认为在 SARS-CoV-2 感染的一些更严重的症状（例如神经元、肺和其他器官损伤）中发挥着主导作用。
我们现在可以为客户提供一系列用于 COVID-19 研究的 NSP 抗体。
目录号TargetImmunogen 目录号Domain29287-1-APSARS-COV-2 NSP1 多克隆抗体Ag309351-180aa29286-1-APSARS-COV-2 NSP5 多克隆抗体Ag309341-306aa29263-1 -APSARS-COV-2 NSP7 多克隆抗体Ag309331-83aa29262-1-APSARS-COV-2 NSP8 多克隆抗体Ag309321-198aa29264-1-APSARS-COV-2 NSP12 多克隆抗体Ag309311-200aa29467-1-APSARS-COV-2 NSP13 多克隆抗体Ag 30930318-469aa29301 -1-APSARS-COV-2 NSP15 多克隆抗体Ag309291-346aa点击此处浏览所有COVID-19相关产品
特色产品辣根过氧化物酶 (HRP) 抗体辣根过氧化物酶 (HRP) 抗体Proteintech 提供 HRP 二抗偶联物，这些抗体已针对各种应用进行了广泛测试和优化。
辣根过氧化物酶 (HRP) 是一种氧化酶，由于其稳定性和底物的多样性，在生物化学中具有广泛的应用。通过使用不同的化学发光和比色底物，HRP 可用于在蛋白质印迹中检测抗原、在 ELISA 中定量目标以及在免疫组织化学中揭示组织中蛋白质的位置。
在下面了解一系列 Proteintech HRP 偶联二抗。
货号产品名称应用反应性验证图像HRP-660056*His，His-Tag 抗体
ELISA，WB重组蛋白6*His，His-Tag 抗体图像HRP-66031α 微管蛋白抗体
ELISA，WB人，小鼠,ratalpha 微管蛋白抗体 imagesHRP-60008beta Actin 抗体
ELISA, WB人类、小鼠、大鼠、斑马鱼、植物β肌动蛋白抗体图像HRP-66002GFP 标签抗体
ELISA, WB重组蛋白, 维多利亚水母GFP 标签抗体图像活动虚拟活动：成功职业规划的关键虚拟活动：成功职业规划的关键立即注册！信息面试：成功职业规划的关键
网络是发现和利用自己与他人之间联系的过程中的重要组成部分。针对这些非正式和正式的社交机会制定有效的策略是职业探索和求职的重要组成部分。在本次研讨会上，我们将重点介绍信息面试作为一种社交工具，当您无法亲自与他人会面时，该工具的效果非常好。
在本次研讨会中，您将学习如何：接近他人您可能认识也可能不认识，以进行信息面试
建立并维持网络关系
利用您的网络进行求职过程
了解您的在线个人资料如何可以在此过程中发挥作用
日期：2020 年 10 月 20 日星期二时间：下午 3-4 点（英国夏令时）/上午 9-10 点（中部时间）注册在线注册现已开放！名额按照先到先得的原则分配。尽早注册，以免失望。
立即注册与演讲者 Dr. 会面。 Briana Konnick，芝加哥大学Briana Konnick 博士是芝加哥大学研究生职业发展副主任，在一个名为 UChicagoGRAD 的中央行政办公室工作。
Briana 为以下人员提供职业和专业发展服务：所有 STEM 项目的博士生和博士后。 Briana 此前曾担任斯克里普斯研究所的项目协调员，为生物医学科学家提供职业支持。她在范德比尔特大学获得本科学位，在斯克里普斯研究所获得生物学博士学位，并在那里进行了短期博士后任命。她目前担任研究生职业联盟 (GCC) 主席，该组织为研究生水平的职业和专业发展提供国际声音领导者。
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Beta Actin Monoclonal antibody Beta Actin Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA Reactivity human, mouse, rat, hamster, zebrafish, monkey, dog and More (9) Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin Proteintech vs. Other VendorsValidation Data Gallery\"Beta Actin Antibodies\" ComparisonView side-by-side comparison of Beta Actin antibodies from other vendors to find the one that best suits your research needs.Compare nowWB analysis of multi-cells using 66009-1-IgWestern blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds).View All Images (17)×Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds).IF Staining of MDCK using 66009-1-IgImmunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).View All Images (17)×Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).WB analysis using 66009-1-IgVarious lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.View All Images (17)×Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.WB analysis of A549 cells using 66009-1-IgA549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.View All Images (17)×A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.IP experiment of HeLa using 66009-1-IgIP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.View All Images (17)×IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.FC experiment of HeLa using 66009-1-Ig1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.View All Images (17)×1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.IHC staining of human kidney using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).View All Images (17)×Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens). -/ - Cat No. 66009-1-Ig Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inmulti-cells, Jurkat cells, A549 cells, CHO cells, HeLa cellsPositive IP detected inHeLa cellsPositive IHC detected inhuman kidney tissue, human brain tissue, human colon cancer tissue, human heart tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inMDCK cells, HepG2 cellsPositive FC detected inHeLa cells Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody. Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:5000-1:50000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WBImmunohistochemistry (IHC)IHC : 1:20-1:2000Immunofluorescence (IF)IF : 1:500-1:2000Sample-dependent, check data in validation data gallery Cited Reactivity Aedes albopictus, Arabidopsis, bovine, canine, chicken, Cyprinus carpio, dog, Drosophila, duck, fish Host / Isotype Mouse / IgG2b Calculated molecular weight 42 kDaObserved molecular weight 42 kDaGenBank accession numberNM_001101 Gene symbol ACTB Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationActins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used \"housekeeping gene\" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.What is the molecular weight of beta-actin? 42 kDaWhat is the function of beta-actin?Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).What are the applications for beta-actin?As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).What are the post-transcriptional modifications (PTMs) to beta actin?Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer\'s disease (PMID: 11246152).For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable. ProtocolsProduct Specific ProtocolsWB protocol for Beta Actin antibody 66009-1-IgDownload protocolIHC protocol for Beta Actin antibody 66009-1-IgDownload protocolIF protocol for Beta Actin antibody 66009-1-IgDownload protocolIP protocol for Beta Actin antibody 66009-1-IgDownload protocolFC protocol for Beta Actin antibody 66009-1-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (2009)KD/KO (1)WB (1949)IHC (3)IF (9)CoIP (1)SpeciesApplicationTitlemouseWBProc Natl Acad Sci U S ACell-autonomous adiposity through increased cell surface GLUT4 due to ankyrin-B deficiency.Authors - Damaris N LorenzoView ArticleratWBMol Med RepFunction of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.Authors - Qian WangView ArticleratWBJ Cell Mol MedNucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity.Authors - Yi GuanView ArticlemouseWBJ DiabetesInhibition of HMGB1 attenuates podocyte apoptosis and epithelial-to-mesenchymal transition (EMT) by regulating autophagy flux.Authors - Juan JinView ArticlehumanWBTheranosticsOncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.Authors - Yaodong ChenView ArticlepigWBArch VirolPorcine circovirus type 2 ORF5 protein induces endoplasmic reticulum stress and unfolded protein response in porcine alveolar macrophages.Authors - Yueling OuyangView Article See more citations ReviewsAdd a reviewThe reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.FHEric (Verified Customer) (06-25-2021)We ordered this actin antibody to detect actin in the cell lysates during the western blot, and it works very well, and the antibody is very sensitive. We highly recommend it.Applications: Western BlotCell Tissue Type: human macrophagesBeta Actin Antibody Western Blot validation ( dilution) in human macrophages (Cat no:66009-1-Ig)FHElisa (Verified Customer) (06-14-2021)This antibody work really well in cells and tissues (mouse). I have also used in human skeletal muscle with good results as well.Applications: Western BlotPrimary Antibody Dilution: 1:5000Cell Tissue Type: C2C12, Heart, skeletal muscle, adipose, kidney, liverBeta Actin Antibody Western Blot validation (1:5000 dilution) in C2C12, Heart, skeletal muscle, adipose, kidney, liver (Cat no:66009-1-Ig)FHAmbra (Verified Customer) (01-20-2021)Works nicely.Used on Human brain lysates (temporal cortex).Applications: Western BlotPrimary Antibody Dilution: 1:2000Cell Tissue Type: Human brain lysateBeta Actin Antibody Western Blot validation (1:2000 dilution) in Human brain lysate (Cat no:66009-1-Ig)FHV (Verified Customer) (01-12-2021)Very good product; signal is goodApplications: Western BlotFHYu Siong (Verified Customer) (07-30-2020)Generate very consistent result for my porcine brain endothelial cells. The antibody at dilution of 1:5000 can be reused for multiple times.Applications: Western BlotPrimary Antibody Dilution: 1:5000Cell Tissue Type: porcine brain endothelial cellsBeta Actin Antibody Western Blot validation (1:5000 dilution) in porcine brain endothelial cells (Cat no:66009-1-Ig)FHMagda (Verified Customer) (12-31-2019)I needed to run a housekeeping gene control for my protein expression test. I was testing HEK293T cell lysates. My Lab did not have a proper AB, but a colleague from a lab next door borrowed me a sample from Proteintech. I was very happy with the results, they were consistent among my triplicate. I would purchase this AB but as a HRP conjugate, as it saves more time and provides cleaner results.Applications: Western Blot,Cell Tissue Type: HEK293TFHElisa (Verified Customer) (10-21-2019)The antibody works very nicely, very clean and I used only 10 uL of protein.Applications: Western Blot,Primary Antibody Dilution: 1:5000Cell Tissue Type: Cell line C2C12FHTracy Chih-Ting (Verified Customer) (09-24-2019)I used this antibody for Hydra actin immunostaining. The outcome is quite good. Applications: Immunofluorescence,Primary Antibody Dilution: 1:500FHRui (Verified Customer) (09-02-2019)Very specific and strong labeling in whole cell lysates.Applications: Western Blot,Primary Antibody Dilution: 1:5000Cell Tissue Type: Cos-7FHHuai-Chin (Verified Customer) (06-19-2019)Very good anitbody. Works well in Western for various type of cells.Applications: Western Blot,Primary Antibody Dilution: 1:3000Cell Tissue Type: Primary cells, various cell linesFHFrancesco (Verified Customer) (06-12-2019)in the testing staining it kind of worked, even if with higher dilution it could work better.anyway further test should be done to confirm it.if results are reproducible it might be a valid alternative to more expensive antibodyApplications: Immunohistochemistry,Primary Antibody Dilution: 1/1500Cell Tissue Type: Brain cellsFHKyosuke (Verified Customer) (06-05-2019)This antibody works very well for Western blot using vascular tissue or cell lysates.Applications: Western Blot,Primary Antibody Dilution: 1/1000Cell Tissue Type: aorta WB FiguresWB analysis of A549 cells using 66009-1-IgA549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.WB analysis of CHO using 66009-1-IgCHO cells were subjected to SDS PAGE followed by western blot with 66009-1-Ig at dilution of 1:25000 incubated at room temperature for 1.5 hours.WB analysis using 66009-1-IgVarious lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.WB analysis using 66009-1-IgVarious lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.WB analysis of multi-cells using 66009-1-IgWestern blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds).IHC FiguresIHC staining of human brain using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human brain using 66009-1-Ig at dilution of 1:500 (under 10x lens).IHC staining of human brain using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human brain using 66009-1-Ig at dilution of 1:500 (under 40x lens).IHC staining of human colon cancer using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66009-1-Ig (beta actin Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human colon cancer using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66009-1-Ig (beta actin Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human heart using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human heart using 66009-1-Ig at dilution of 1:500 (under 10x lens).IHC staining of human heart using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human heart using 66009-1-Ig at dilution of 1:500 (under 40x lens).IHC staining of human kidney using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 10x lens).IHC staining of human kidney using 66009-1-IgImmunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).IP FiguresIP experiment of HeLa using 66009-1-IgIP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.IF FiguresIF Staining of HepG2 using 66009-1-IgImmunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66009-1-Ig(beta Actin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IF Staining of MDCK using 66009-1-IgImmunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).FC FiguresFC experiment of HeLa using 66009-1-Ig1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. \"Beta Actin antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare. WB : 1:5000-1:50000IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WBIHC : 1:20-1:2000IF : 1:500-1:2000 human, mouse, rat, hamster, zebrafish, monkey, dog, aedes albopictus, arabidopsis, bovine, canine, chicken, cyprinus carpio, drosophila, duck, fish Pig, Hirudo Medicinalis, Bovine, Rat, Canine, Feline, Human, Rabbit, Carp, Mouse, Guinea Pig, Chicken, Sheep
