Anti-GST (GST.B6) Mouse Monoclonal Antibody

Additional Information

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Anti-GST

Anti-GST (GST.B6) Mouse Monoclonal Antibody

Y1021

100 µg

~55/25 kDa

N/A

Immunogen: GST protein

10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)

1 µg/µl

WB (with ECL): 10-50 ng of purified GST or GST fusion proteins

Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.

PDF Datasheet， MSDS

N/A

(Click image to enlarge)1:5000 (0.2 μg/ml) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein

Wet ice shipping

N/A

Details

SpecificityRecognizes native and denatured forms of purified GST or GST fusion proteinsIsotypeMouse IgG2a CloneGST.B6PurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IS, IP, WBvSensitivityWB (with ECL): 10-50 ng of purified GST or GST fusion proteinsWorking ConditionsWB (with ECL) 1:1000-3000 dilutionIS: 1:500-2000 dilutionFor best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)1:5000 (0.2 μg/ml) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein