This kit is designed to determine DUB inhibitor specificity and potency. You can select 10 DUBs of your choice from our DUB list. Each DUB is sufficient for 20 X 50ul reactions using a working concentration of 10nM. The supplied Ub-rhodamine 110 is sufficient for 550 X 50ul reactions using a working concentration of 100nM. The supplied iodoacetamide can be used as an inhibitor of cysteine DUBs using a working concentration of 10mM.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Deubiquitinating Enzyme Inhibitor Profiling Kit I

N/A

J6110

1 Kit

N/A

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

90% - 95% based on SDS-PAGE

Store at -80°C; avoid multiple freeze-thaw cycles

PDF datasheet

N/A

N/A

Dry ice shipping

N/A

Details

* When place an order, please select any ten (10) DUBs from the list on the right side of this page.DescriptionThis kit is designed to determine DUB inhibitor specificity and potency. You can select 10 DUBs of your choice from our DUB list. Each DUB is sufficient for 20 X 50ul reactions using a working concentration of 10nM. The supplied Ub-rhodamine 110 is sufficient for 550 X 50ul reactions using a working concentration of 100nM. The supplied iodoacetamide can be used as an inhibitor of cysteine DUBs using a working concentration of 10mM.Components• 10X DUBs (pick 10 DUBs from the list, 200nM)..................50 µl each• Ub-rhodamine 110 (1mg/ml)............................................................25 µl • Iodoacetamine (IAA).........................................................................25 mg• 5X Deubiquitination Buffer.........................................................2X1.25mlDUBs List1. 6xHis-Uch372. Adrm1/Uch373. 6xHis-Usp144. UCHL15. GST-UCHL16. UCHL37. GST-UCHL38. GST-Ataxin3(Q22)9. OTUB110. GST-OTUB111. OTUB212. GST-OTUB213. GST-A20(1-365)14. OTUD215. OTUD6B16. 6xHis-OTULIN17. GST-USP418. USP719. GST-USP720. GST-USP9X (catalytic domain)21. GST-USP1122. GST-USP1523. GST-USP21(195-565)24. GST-USP30(57-517)