Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Ub-AMC

Ubiquitin-AMC

M3010

50 µg

9 kDa

Human

Bacterial recombinant

DMSO

See tube label

>95% by SDS-PAGE

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet

N/A

No

Dry ice shipping

Wilkinson KD, et al. (2005) Methods Enzymol. 399, 37-51

Details

Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively. Images(Click image to enlarge)Coomassie-stained SDS-PAGE5 µg purified Ub-AMC The reaction contained 2 nM bovine 26S proteasome and 1 mM Ub-AMC. AMC fluorescence was monitored by a plate reader using excitation and emission filter set at 360/40 and 460/30 nm, respectively.