Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K27R), lysine 27 was substituted with an arginine, thereby it cannot form lysine 27-linked polyubiquitin chains.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

6xHis-Ubiquitin (K27R)

6xHis-UbK27R

E1740

1 mg

8.5 kDa

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~95% by SDS-PAGE

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

N/A

No

Dry ice shipping

1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM (1997) FASEB 11(13), 1055 – 1066.3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228.

Details

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K27R), lysine 27 was substituted with an arginine, thereby it cannot form lysine 27-linked polyubiquitin chains.Images(Click image to enlarge)Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified 6xHis-Ubiquitin (K0)Lane 3: 5 µg purified 6xHis-Ubiquitin (K6R)Lane 4: 5 µg purified 6xHis-Ubiquitin (K11R)Lane 5: 5 µg purified 6xHis-Ubiquitin (K27R)Lane 6: 5 µg purified 6xHis-Ubiquitin (K29R)Lane 7: 5 µg purified 6xHis-Ubiquitin (K33R)Lane 8: 5 µg purified 6xHis-Ubiquitin (K48R)Lane 9: 5 µg purified 6xHis-Ubiquitin (K63R)