Class II fluorescence polarization HTS reagent based on peptide sequence 56–69 of ubiquitin. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys63 to Ub via a native isopeptide bond. See references 4-6 for full experimental and analytical details.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

TAMRA-Ub56-69(K63)-Ub

N/A

M4070

1 mg

10.61 kDa

Human

Synthetic

Lyophilized powder

N/A

≥95% by RP-HPLC and SDS-PAGE (CBB and fluorescence sacnning). Protein content verified by SDS-PAGE.

Store powder and DMSO stock at −20°C, aqueous stocks at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.

PDF Datasheet， MSDS

N/A

N/A

Wet ice shipping

1. Tirat et al. Anal. Biochem. 2005, 343, 244. 2. Huang et al. Methods in Molecular Biology 2009, 565, 127. 3. Levine et al. Anal. Biochem. 1997, 247, 83. 4. Faesen et al. Chem. Biol. 2011, 18, 1550. 5. Geurink et al. ChemBiochem 2012, 13, 293. 6. Mevissen et al. Cell 2013, 154, 169.

Details

Class II fluorescence polarization HTS reagent based on peptide sequence 56–69 of ubiquitin. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys63 to Ub via a native isopeptide bond. See references 4-6 for full experimental and analytical details.