IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in _{E. coli}. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate.

Additional Information

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IPTG Solution (1M)

isopropyl - β - D - thiogalactopyranoside

P1011-100

100 ml

367-93-1

C9H18O5S

238.3

Liquid. Sterilized by filtration through a 0.22 micron filter.

>99% by HPLC, dioxane-free

Synthetic

Eligible for ambient temperature shipping. Store at 4°C upon receiving.

PDF Datasheet

Wet ice shipping

J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.

Details

IPTG, Isopropyl β-D-1-thiogalactopyranoside, binds the lac repressor and induces expression of β-galactosidase in _{E. coli}. IPTG is widely used to induce the expression of cloned genes under the control of the lac operon and to screen blue/white colony in X-gal plate. Expression of GST-UCHL3 in BL21 (DE3) cells was induced by the addition of 25 μM (lane 3), 100 μM (lane 4), 200 μM (lane 5) or 400 μM (lane 6) IPTG at 37 0C for 2 hours. Lane 2 without IPTG.