Deubiquitinating enzymes (DUBs) are proteases that posses the ability to cleave ubiquitin chains or the isopeptide bond that conjugates ubiquitin with a substrate. Human cells have approximately 100 DUBs that play important roles in regulating various cellular events. OTUB1 is a member of the ovarian tumor domain family. It is recognized as a K48 ubiquitin chain-specific DUB and can inhibit E2 enzymes, especially Ubc13 (also known as Ube2N), which is known to synthesize K63-linked ubiquitin chains. OTUB1 may also deubiquitinate TRAF3 and -6. This would negatively regulate virus-triggered type I IFN induction.
Additional Information
Product Name: | GST-OTUB1 |
Also Known As: | OTB1; OTU1; HSPC263 |
Catalog No.: | H3011 |
Size: | 100 µg |
Molecular Weight: | 31.2 kDa |
Species: | Human |
Source: | Bacterial recombinant |
Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
Concentration: | See tube label |
Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image |
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF Datasheet， MSDS |
NCBI RefSeq: | NM_017670 |
Image(s): | N/A |
Shipping Method: | Dry ice shipping |
References: | 1. Balakirev MY, et al. (2003) EMBO Rep 4(5), 517 – 522.2. Wang T, et al.(2009) J Mol Biol 386(4), 1011 – 1023.3. Reyes-Turcu FE, et al. (2009) Annu Rev Biochem 78, 363 – 397.4. Li S, et al. (2010) J Biol Chem 285(7), 4291 – 4297.5. Wiener R, et al. (2012) Nature 483(7391), 618 – 622. |
Details
Deubiquitinating enzymes (DUBs) are proteases that posses the ability to cleave ubiquitin chains or the isopeptide bond that conjugates ubiquitin with a substrate. Human cells have approximately 100 DUBs that play important roles in regulating various cellular events. OTUB1 is a member of the ovarian tumor domain family. It is recognized as a K48 ubiquitin chain-specific DUB and can inhibit E2 enzymes, especially Ubc13 (also known as Ube2N), which is known to synthesize K63-linked ubiquitin chains. OTUB1 may also deubiquitinate TRAF3 and -6. This would negatively regulate virus-triggered type I IFN induction.
