This kit is designed for enrichment of cellular proteins conjugated with lysine 29/33-linked polyubiquitin chains using GST-TRABID (ZNF1). Polyubiquitinated proteins bound on GST-TRABID (ZNF1) can be precipi-tated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Enriched polyubiqui-tinated proteins can be assayed by immunoblotting or mass spectrometry.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s):

K29/K33 Polyubiquitin Chain Capture Kit

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J4470

1 Kit

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Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

Enzyme >95% by SDS-PAGE

Store at -80°C; avoid multiple freeze-thaw cycles

PDF datasheet

N/A

Images:(Click image to enlarge)

A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates

GST-TRABID(NZF1) pulldown assay1. 20 ug cell lysates2. GST pulldown3. GST-TRABID(NZF1) pulldown

Shipping Method: Dry ice shipping References: N/A

Details

Description This kit is designed for enrichment of cellular proteins conjugated with lysine 29/33-linked polyubiquitin chains using GST-TRABID (ZNF1). Polyubiquitinated proteins bound on GST-TRABID (ZNF1) can be precipi-tated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Enriched polyubiqui-tinated proteins can be assayed by immunoblotting or mass spectrometry. Components • GST-TRABID (ZNF1) (2 mg/ml each)......................................1 ml • GST (4 mg/ml)..........................................................................0.5 ml • Iodoacetamine (IAA).................................................................25 mg • Glutathione (reduced)..............................................................100 mg• Glutathione Agarose Resin (75% slurry)..................................1.0 ml Images:(Click image to enlarge) A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates GST-TRABID(NZF1) pulldown assay1. 20 ug cell lysates2. GST pulldown3. GST-TRABID(NZF1) pulldown