Ubiquitin (+1) consists of the first 75 amino acids of ubiquitin, but lacks glycine 76 because of the deletion of two of the three nucleotides that encode glycine 76. The resulting frame shift leads to the addition of 20 amino acids (YADLREDPDRQDHHPGSGAQ) after glycine 75. Ubiquitin (+1) can not support ubiquitination reactions. When expressed at high levels, it could inhibit proteasomal degradation. Ubiquitin (+1) was also found in protein aggregates in patient brains of Alzheimer’s disease and other neurodegenerative diseases.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Ubiquitin (+1)

Ub (+1)

E1120

250 µg

10.8 kDa

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~95% by SDS-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

N/A

(Click image to enlarge)

Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified GST-Ubiquitin (+1)Lane 2: 5 µg purified Ubiquitin (+1)

Dry ice shipping

1. van Tijn, P. et al. (2007) J. Cell Sci. 120:1615.2. van Leeuwen FW, et al. (2002) Int J Biochem Cell Biol. 34(11):1502-5.

Details

Ubiquitin (+1) consists of the first 75 amino acids of ubiquitin, but lacks glycine 76 because of the deletion of two of the three nucleotides that encode glycine 76. The resulting frame shift leads to the addition of 20 amino acids (YADLREDPDRQDHHPGSGAQ) after glycine 75. Ubiquitin (+1) can not support ubiquitination reactions. When expressed at high levels, it could inhibit proteasomal degradation. Ubiquitin (+1) was also found in protein aggregates in patient brains of Alzheimer’s disease and other neurodegenerative diseases.Images:(Click image to enlarge) Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified GST-Ubiquitin (+1)Lane 2: 5 µg purified Ubiquitin (+1)