Loading Control Antibody Kit contains three commonly-used loading control antibodies. The anti-GAPDH, beta-actin and beta-tubulin antibodies in this kit are mouse monoclonal antibodies purified by protein A affinity chromatography from mouse ascites fluids. You save approximately 30% compared to buy individually.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

Loading Control Antibody Kit

N/A

J5200

1 Kit

~55/25 kDa

Human

No

10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)

1 µg/µl

Guaranteed for detecting endogenous loading control proteins in 20 µg cell or tissue lysates

Store at -20°C or -80°C for one year. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.

PDF Datasheet， MSDS

N/A

No

Wet ice shipping

N/A

Details

Loading Control Antibody Kit contains three commonly-used loading control antibodies. The anti-GAPDH, beta-actin and beta-tubulin antibodies in this kit are mouse monoclonal antibodies purified by protein A affinity chromatography from mouse ascites fluids. You save approximately 30% compared to buy individually.ComponentsAnti-GAPDH (Clone# GA1R): 100 µgAnti-beta-Actin (Clone# BA3R): 100 µgAnti-beta-Tubulin (Clone# BT7R): 100 µgPurificationProtein A affinity chromatography from mouse ascites fluidApplicationsDot, ELISA, IS, WBWorking ConditionsFor Western Blot with ECL, Anti-GAPDH 1:1000-10,000 dilution; Anti-beta-Actin 1:1000-5,000 dilution; Anti-beta-Tubulin 1:1000-5,000 dilution.For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration testImages(Click image to enlarge)Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)