Antigen/Purification: Collapse
The antigen is a synthetic peptide taken from the C-terminus of rat clavesin 1.
The antibody is prepared from serum by affinity purification using a column to which the peptide antigen was coupled.
Biological Significance: Collapse
Clavesin (clathrin vesicle associated Sec14 protein) is a novel neuron specific protein that has recently been identified and shown to be required for normal morphology of late endosomes and/or lysosomes as lentiviral-mediated knockdown of clavesin in hippocampal neurons causes lysosomal defects (Katoh et al., 2009). Additionally, upregulation of clavesin in human hepatocellular carcinoma has recently been demonstrated thus making it a useful marker for this disease state (Zhao et al., 2008).
Storage
100 µl in PBS + 50% glycerol.
For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year after date of receipt. Avoid freeze/thaw cycles.
General References
Yohei Katoh, Brigitte Ritter, Thomas Gaffry, Francois Blondeau, Stefan Höning and Peter S. McPherson (2009) the clavesin family: neuron-specific lipid- and clathrin-binding Sec14 proteins regulating lysosomal morphology. Journal of Biological Chemistry Oct 2;284(40):27646-54.
Zhao S, Xu C, Qian H, Lv L, Ji C, Chen C, Zhao X, Zheng D, Gu S, Xie Y, Mao Y (2008) Cellular retinaldehyde-binding protein-like (CRALBPL), a novel human Sec14p-like gene that is upregulated in human hepatocellular carcinomas, may be used as a marker for human hepatocellular carcinomas. DNA Cell Biol. Mar; 27(3):159-63.
