Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4 C. For long-term storage, aliquot and store at -20 C or below. Avoid multiple freeze-thaw cycles. Recombinant protein encompassing a sequence within the C-terminus region of human c-ABL. The exact sequence is proprietary. ABL proto-oncogene 1, non-receptor tyrosine kinase , ABL , CHDSKM , JTK7 , bcr/abl , c-ABL , c-ABL1 , p150 , v-abl The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq] Various whole cell extracts (30 g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (GTX111317) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Immunohistochemical analysis of paraffin-embedded human hepatoma, using c-Abl(GTX111317) antibody at 1:500 dilution.Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min c-Abl antibody detects c-Abl protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Abl protein stained by c-Abl antibody (GTX111317) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 m. Whole cell extract (30 g) was separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (GTX111317) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778 Articles 五大验证策略 GeneTex employs various analytic validation strategies to ensure both consistent quality and specificity.