Microarray E. coli Total RNA Labeling for High Density Oligonucleotide Array [PDF] Labeling E. coli total RNA for high density oligonuceotide array like Affymetrix E. coli antisense genome array. [updated/corrected 05/30/2007] E. coli Total RNA Isolation [PDF] Stablize E. coli RNA with Qiagen Bacteria RNA Protect Reagent and isolate total RNA with Epicentre MasterPure RNA Purification Kit. [updated 07/31/2003] E. coli Total RNA Labeling for Spotted Microarray [PDF] Label E. coli Total RNA with CyDye-dUTP directly using Invitrogen Superscript II reverse transcriptase. [updated/corrected 05/30/2007] E. coli Genomic DNA Labeling [PDF] Label fragmented E. coli genomic DNA with CyDye-dUTP directly using Klenow Fragment. [updated 02/10/2002] Spotted array Hybridization [PDF] Hybridize CyDye-dUTP labeled probes to E. coli microarray printed on poly-lysine coated slide (provided by GEC) using Sigma ArrayHyb Buffer. [updated 02/10/2002] Mutagenesis Tn5 Mutagenesis [PDF] Mutate E. coli K-12 genes with Tn5 Transposon. [updated 05/11/2002] Media These are our protocols for preparing Neidhardt\'s MOPS-based defined media. These media are used for the global transcription analyses, and are now available commercially from TekNova Inc. EZ Rich Defined Medium [PDF] MOPS Minimal Medium [PDF]