Species: Human Host Species: Sf9 insect cells MW: 51 kDa Genbank #: NM_022750 Tag(s): N-terminal GST-tag a.a: 500–701(end) Description:Human PARP12 (GenBank Accession No. NM_022750), a.a. 500-701(end) with N-terminal His-GST-tag, MW = 51 kDa, expressed in a Baculovirus infected Sf9 cell expression system. UniProt Q9H0J9 Synonym(s): poly (ADP-ribose) polymerase 12, poly(ADP-ribosyl)transferase 12, PARP12 Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Assay Conditions: Ribosylationreaction is conducted in wells coated with amixture of histones at room temperature for 1hour. Assay buffer: 50 mM Tris-HCl (pH 8.0),5 mM MgCl₂ and 20 µM ZnCl₂ . Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 8 mM glutathione, 0.04% Tween-20, and 20% glycerol. Format: Aqueous buffer solution Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Katoh,M. and Katoh,M. Int. J. Oncol. 23 (2), 541-547 (2003). 2. Hillier,L.W., et al. Nature 424 (6945), 157- 164 (2003). Warning(s): Avoid freeze/thaw cycles. Scientific Category: PARP Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.