This monoclonal antibody enables sensitive and specific detection of human IgG in immunoassays such as ELISA and ELISpot.

We recommend biotinylated MT391 as detection antibody in ELISpot in combination with capture antibody MT134. In antigen-specific ELISA and ELISpot, MT391 is recommended for detection in combination with coated antigen. See Related products.

MT391 is also available conjugated to ALP (MT391-ALP) for use in sandwich ELISA. See Related products.

Analyte Information

IgGIgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. In mice the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3. The IgG molecule consists of two heavy and two lights chains (κ or λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.