This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock™ reagent and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock™, a non-radioactive, superior phosphate detection reagent.

Key features of PiColorLock™ include:

• Colorimetric Assay – competitor assays are radioactive
• Special additive ensures low backgrounds with acid-labile substrates
• Unparalleled stability of phosphate-dye complexes
• Reagent is compatible with almost any assay buffer
• No inhibition of color development by high concentrations of protein
• Stable reagent formulation – long shelf life

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Comparison with competitors:

Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color- see below).