RNA Interference Targeting ��21����ene of Pedicle Periosteum Cells from Sika Deer by shRNA Lentivirus\" />antler regeneration,P21gene,RNAi,pedicle periosteum,lentiviral expression vector��,\"/> [an error occurred while processing this directive]|[an error occurred while processing this directive] 1.���տƼ���ѧ, �� 212018;��2.�й�ũҵ��ѧԺ�ز��о��� ����ʡ���־��ö����������ѧ����ص�ʵ����������, ���� 130112�� RNA Interference Targeting ��21����ene of Pedicle Periosteum Cells from Sika Deer by shRNA Lentivirus GUO Qian��qian1,2��, WANG Da��tao2, CHU Wen��hui2,��LU Xiao��ping2, QIN Xin1,2��, ZHAO Hai��ping��2, LI Chun��yi2 1.Jiangsu University of Science and Technology, Zhenjiang 212018, China; 2. State Key Laboratory ��ultivation Base of Jilin Provincial ��pecial ��conomical Animal Molecular Biology, Institute of Special Animal and Plant����ciences of CAAS, Changchun 130112, China ժҪ���������ϵͳ,�Զ���÷��¹�DZ��Ĥ��ϸ��(PPϸ��)P21������и��š�������:ɸѡ����2�����÷��¹P21�����siRNA����������PLVTHM���ӳɹ�,����pMD2.G��pCMV��dr8��9������תȾ��293tϸ��,���������;ͨ���ȾPPϸ��������ʽϸ���ǽ��з�ѡ,����˴���90�����ϵĸ�Ⱦϸ��;ӫ�ⶨ��RT-PCR������P21�����mRNAˮƽ�����µ�,����Ч�ʴﵽ70���������ɹ�������P21������PPϸ���еı��,����˵ͱ��P21��PPϸ��ϵ�� Abstract��P21 gene of the pedicle periosteal cells of sika deer was interfered using RNAi in lentiviral vector system. The results showed that: (1) Two sequences of small interfering RNAs, targeting P21 gene of sika deer, were successfully reassembled into the lentiviral plasmids(Plvthm). Positive clones were identified based on the results of both PCR and sequencing. Recombinant lentivirus was acquired by each positive plasmid co��transfecting into 293 T cells with the plasmids pMD2.G and pCMV-dr8��9; (2) Recombinant lentivirus was successfully interfered into the PP cells, and the GFP positive cell proportion obtained by flow cytometry (FCM) sorting was about 90��; (3) The result of RT-PCR showed that the expression level of P21 mRNA in cells infected with recombinant lentiviral was obviously decreased, and the interferential efficiency was about 70��.Therefore, in this study, we successfully interfered the expression of P21 gene in the pp cells, and obtained pp cell line with decreased expression of P21 gene, which lays foundation for revealing the regulatory mechanism of P21 underlying antler regeneration. ��ٻٻ1,2��,��������2��. ���������������÷��¹�DZ��Ĥϸ��P21����[J]. ����ũҵ��ѧѧ��, 2014, 36(1): 116-121. ��ï�Σ��ں��������骣���־�ԣ������«����. ţFADD��������RNAi���幹������ţ̥�����άϸ���еı��[J]. ����ũҵ��ѧѧ��, 2014, 36(2): 199-204.