内切酶活性表-蚂蚁淘生物

EnzymeSuppliedNEBuffer%ActivityinNEBuffers

1	2	3	4
AATII	4	0	50	50	100
AccI	4	50	50	10	100
Acc65I	3+BSA	10	75	100	25
AciI	3	25	50	100	50
AclI	4+BSA	10	10	0	100
AfeI*	SE-Y	25	50	25	100
AflII	2+BSA	50	100	25	100
AflIII	3+BSA	25	75	100	50
AgeI	1	100	50	10	75
AhdI	4+BSA	25	75	0	100
AleI	4	10	20	10	100
AluI	2	100	100	75	100
AlwI	4	50	100	10	100
AlwNI	4	50	100	50	100
ApaI@25°C	4+BSA	25	50	0	100
ApaLI	4+BSA	100	100	10	100
ApoI@50°C	3+BSA	10	75	100	75
AscI	4	0	10	10	100
AseI	3	NR	75	^dd100	NR
AsiSI	3+BSA	50	100	100	50
AvaI	4	10	75	10	100
AvaII	4	50	75	10	100
AvrII	2	100	100	50	100
BaeI@25°C	2+BSA+SAM	50	100	50	75
BamHI	U^dd+BSA	75	100	50	75
BanI	4	50	100	50	100
BanII	4	100	10	50	100
BbsI	2	100	100	25	75
BbvI	2	100	100	25	75
BbvCI	4	50	100	10	100
BceAI	U^dd+BSA	25	NR	NR	75
BcgI	U+SAM	50	75	100	50
BciVI	4	100	25	10	100
BclI@50°C	3	50	100	^{^dd}100	75
BfaI	4	75	50	10	100
BfrBI	4+BSA	10	25	10	100
BfuAI@50°C	3	0	75	100	10
BfuCI	4+BSA	100	50	25	100
BglI	3	50	75	100	50
BglII	3	10	75	100	10
BlpI	4	50	100	10	100
Bme1580I	2	75	100	75	75
BmgBI	3+BSA	0	25	100	10
BmrI	2	75	100	75	100
BmtI*	SE-W	25	100	10	50
BpmI	3+BSA	75	100	100	100
Bpu10I*	SE-K	0	25	100	25
BpuEI@25°C	2+SAM	75	100	10	75
BsaI@50°C	3	75	75	100	100
BsaAI	3	75	75	100	75
BsaBI@60°C	2	75	100	100	100
BsaHI	4+BSA	50	100	100	100
BsaJI@60°C	2	100	100	100	100
BsaWI@60°C	2+BSA	50	100	50	50
BsaXI	4	75	100	10	100
BseRI	2	100	100	75	100
BseYI	3	10	50	100	50
BsgI	4+SAM	50	75	50	100
BsiEI@60°C	2+BSA	50	100	10	100
BsiHKAI@65°C	3+BSA	50	100	100	100
BsiWI@55°C	3	100	100	100	25
BslI@55°C	3	10	50	100	75
BsmI@65°C	2	75	100	75	75
BsmAI@55°C	3	50	100	100	50
BsmBI@55°C	3	75	100	100	100
BsmFI@65°C	4+BSA	25	50	50	100
BsoBI@65°C	2	10	100	100	50
Bsp1286I	4+BSA	25	25	25	100
BspCNI@25°C	1+BSA+SAM	100	75	10	75
BspDI	4	25	75	50	100
BspEI	3	0	10	100	0
BspHI	4	10	100	50	100
BspMI	3	NR	NR	100	NR
BsrI@65°C	3	0	50	100	10
BsrBI	2	50	100	100	100
BsrDI@60°C	2+BSA	50	100	50	75
BsrFI	U	25	100	75	75
BsrGI	2+BSA	25	100	10	100
BssHII@50°C	3	100	100	^dd100	100
BssKI@60°C	3+BSA	0	50	100	50
BssSI	3	0	50	^{^dd}100	10
BstAPI*@60°C	SE-W	25	25	100	25
BstBI@65°C	4	75	50	25	100
BstEII@60°C	3	50	75	100	75
BstF5I*@65°C	SE-Y	75	50	25	100
BstNI@60°C	2+BSA	10	100	100	75
BstUI@60°C	2	100	100	50	100
BstXI@55°C	3	25	100	100	50
BstYI@60°C	2	50	100	75	100
BstZ17I	3	NR	NR	100	NR
Bsu36I	3+BSA	0	25	100	0
BtgI	3+BSA	25	50	100	100
BtsII	4+BSA	100	50	50	100
Cac8I	3	50	75	100	75
ClaI	4+BSA	10	50	50	100
DdeI	3	75	100	100	75
DpnI	4	100	100	75	100
DpnII	U	NR	NR	^dd100	NR
DraI	4	75	75	50	100
DraIII	3+BSA	100	75	100	25
DrdI	4	25	50	10	100
EaeI	1	100	100	50	100
EagI	3	10	25	100	10
EarI	1	100	100	50	100
EciI	2+BSA	50	100	100	50
EcoNI	4	100	100	75	100
EcoO109I	4+BSA	100	100	75	100
EcoRI	U^dd	100	100	100	100
EcoRV	3+BSA	50	75	100	50
FatI*	SE-G	10	100	50	50
FauI*@55°C	SE-B	100	25	10	50
Fnu4HI	4	10	25	25	100
FokI	4	50	100	75	100
FseI	4+BSA	100	75	0	100
FspI	4	10	75	10	100
HaeII	4+BSA	75	100	50	100
HaeIII	2	50	100	25	75
HgaI	1	100	75	50	100
HhaI	4+BSA	75	100	100	100
HincII	3+BSA	75	100	100	100
HindIII	2	50	100	10	50
HinfI	2	75	100	75	75
HinP1I	2	100	100	100	100
HpaI	4	25	50	10	100
HpaII	1	100	50	10	100
HphI	4	NR	75	0	100
Hpy99I	4+BSA	10	10	0	100
Hpy188I	4	50	25	10	100
Hpy188III	4+BSA	100	100	10	100
HpyCH4III	4	100	50	25	100
HpyCH4IV	1	100	25	10	25
HpyCH4V	4	50	50	25	100
I-CeuI	4+BSA	10	10	0	100
I-SceI	U+BSA	10	50	50	50
KasI	2+BSA	75	100	75	75
KpnI	1+BSA	100	75	0	50

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MboI	3	75	100	100	100
MboII	2	100	100	50	100
McrBC	2+BSA+GTP	50	100	10	75
MfeI	4	75	50	10	100
MluI	3	25	75	100	50
MlyI	4+BSA	50	50	25	100
MmeI	4+SAM	NR	NR	NR	100
MnlI	2+BSA	75	100	50	75
MscI	4	75	75	75	100
MseI	2+BSA	75	100	75	75
MslI	2	100	100	25	100
MspI	2	75	100	50	50
MspA1I	4+BSA	50	100	50	100
MwoI@60°C	U	10	75	100	75
NaeI	1	100	75	10	75
N.AlwI	2	25	100	25	50
NarI	1	100	75	75	75
N.BbvCIA	2	50	100	10	100
N.BbvCIB	2	50	100	10	100
N.BstNBI@55°C	U	0	10	10	10
NciI	4	100	25	10	100
NcoI	4	100	100	100	100
NdeI	4	75	100	75	100
NgoMIV	4	100	50	10	100
NheI	2+BSA	100	100	10	100
NlaIII	4+BSA	25	25	25	100
NlaIV	4+BSA	10	10	10	100
NotI	3+BSA	0	75	100	50
NruI	U	0	10	75	10
NsiI	U	25	25	50	10
NspI	2+BSA	100	100	0	100
PacI	1+BSA	100	75	10	100
PaeR7I	4	25	100	10	100
PciI*	SE-O	25	75	100	50
PflFI	4+BSA	0	25	25	100
PflMI	3+BSA	0	100	100	50
PI-PspI@65°C	U+BSA	0	10	10	10
PI-SceI	U+BSA	0	0	0	0
PleI	4	100	100	75	100
PmeI	4+BSA	0	50	10	100
PmlI	1+BSA	100	75	0	75
PpuMI	4	0	25	0	100
PshAI	4+BSA	50	75	10	100
PsiI*	SE-B	50	100	0	25
PspGI@75°C	2	75	100	50	100
PspOMI*	SE-Y	25	100	50	100
PstI	3+BSA	75	75	100	50
PvuI	3+BSA	10	75	100	10
PvuII	2	100	100	100	100
RsaI	1	100	100	50	100
RsrII	4	25	75	10	100
SacI	1+BSA	100	50	10	100
SacII	4	25	75	10	100
SalI	U+BSA	0	0	50	0
SapI	4	75	50	0	100
Sau3AI	U+BSA	100	50	10	100
Sau96I	4	50	100	100	100
SbfI*	SE-Y	75	50	0	100
ScaI	U	25	75	^dd50	25
ScrFI	4	100	100	100	100
SexAI	3+BSA	25	25	100	100
SfaNI	3	0	75	100	25
SfcI	4+BSA	75	50	10	100
SfiI@50°C	2+BSA	0	100	10	100
SfoI	2	25	100	50	50
SgrAI	4	100	50	10	100
SmaI@25°C	4	0	0	0	100
SmlI@55°C	4+BSA	25	75	25	100
SnaBI	4+BSA	25	50	25	^dd100
SpeI	2+BSA	75	100	25	75
SphI	2	100	100	50	100
SspI	U	50	100	50	50
StuI	2	100	100	50	100
StyI	3+BSA	25	75	100	10
StyD4I	2	10	100	100	100
SwaI@25°C	3+BSA	10	10	100	10
TaqI@65°C	U+BSA	50	75	75	75
TfiI@65°C	3	100	100	^dd100	100
TliI@75°C	3+BSA	25	25	100	10
TseI@65°C	3	75	100	100	75
Tsp45I@65°C	1+BSA	100	25	0	75
Tsp509I@65°C	1	100	100	100	NR
TspRI@65°C	4+BSA	25	50	25	100
Tth111I@65°C	1	100	25	25	100
XbaI	2+BSA	0	100	75	75
XcmI	2	10	100	50	50
XhoI	2+BSA	75	100	100	100
XmaI	4+BSA	25	50	0	100
XmnI	2+BSA	100	100	75	100
ZraI*	SE-B	100	100	25	100

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