Mouse Anti-MICA/FITC 流式抗体说明书

Catalog Number : RS-0832R-FITC

Quantity size : 0.1ml（dilute with pH 7.40.01 M PBS or antibody diluent ）

Background: The MHC class I chain-related （MIC） proteins are related to the Major histocompatibility complex （MHC） class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related （MIC） proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

Function : Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

MICA/FITC 流式抗体说明书Specificity : · anti-MICA/FITC is a mouse monoclonal antibody unconjugated

· specific for MICA/FITC of pig

· use for western blotting,elisa,immunoprecipitation and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 43kDa

Application: ·Flow-Cyt=1:50-200

· IF=1:50-200

· Optimal working dilutions must be determined by the end user.

MICA/FITC 流式抗体说明书Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.