protoporphyrin ferrochelatase (cyanobacterial)-蚂蚁淘生物

货号：	AS10 693
抗体名：	兔抗集胞藻原卟啉亚铁鳌合酶多克隆抗体
抗体英文名：	HemH\|protoporphyrin ferrochelatase (cyanobacterial)
浓度：	见说明书
应用范围：	western blot (WB)
宿主：	兔
适应物种：	植物
标记物：	无
克隆性：	否
保存条件：	低温
亚型：	IgG
规格：	100 µl

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HemH|protoporphyrin ferrochelatase (cyanobacterial)介绍：

货号：AS10 693

中文名称：兔抗集胞藻原卟啉亚铁鳌合酶多克隆抗体

英文名称：HemH|protoporphyrin ferrochelatase (cyanobacterial)

应用：western blot (WB)

规格：100 µl

价格：5175元

HemH|protoporphyrin ferrochelatase (cyanobacterial)简介：

PRODUCT INFORMATION
background		HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.
immunogen		KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence
host		rabbit
clonality		polyclonal
purity		serum
format		lyophilized
quantity		100 µl
reconstitution		For reconstitution add 100 µl of sterile water
storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications		western blot (WB)
related products		AS07 228 \| HemH \| protoporphyrin ferrochelatase (reacts with higher plants)
additional information		to be added when available

APPLICATION INFORMATION
recommended dilution		1: 3000 with standard ECL, West pico (WB)
expected \| apparent MW		46 kDa
confirmed reactivity		Synechocystis sp. PCC 6803
predicted reactivity		cyanobacteria
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		loads higher than 0.5 µg of chlorophyll per well are not recommended
selected references		to be added when available

application example

Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon, Czech Republic

$\"western$

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