Human IgE for Mast Cell Degranulation Assays Cat. No. 250206 Human IgE (biotinylated) is successfully used to sensitize human mast cells and trigger degranulation when streptavidin is added to the cell culture media. Human IgE products have been used to stimulate: human mast cellshuman airway smooth muscle cells (HASM)human bronchial/tracheal smooth muscle cells (B/TSM)acute myelogenous leukemia blasts (AML)human neutrophils | | Biotinylated human IgE and streptavidin trigger degranulation of LAD2 human mast cells.LAD2 cells were sensitized overnight with 0.1 ug/ml biotinylated human IgE (Abbiotec Cat. No. 250206). Cells were incubated with streptavidin (SA) for 30 min at 37°C. Degranulation was monitored by beta-hexosaminidase release into the supernatant calculated as a percentage of total content.Data kindly provided by Prof. Fernando de Mora, Universidad Autonoma de Barcelona, Spain. |
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