一种 75 kDa 葡萄糖调节蛋白 (GRP75) 是一种线粒体基质蛋白，通常被认为是热休克蛋白 70 (HSP70) 类蛋白质的成员。 GRP75 在低葡萄糖和其他营养和环境应激条件下被诱导。已在免疫系统（PBP74；CSA）、肝脏（mtHSP70）和细胞系中鉴定出密切相关的蛋白质。 GRP75 参与线粒体中蛋白质易位、折叠和功能的各种伴侣功能。它还似乎参与抗原识别、细胞增殖和衰老。虽然主要定位于线粒体，但相关形式可能存在于胞质溶胶或细胞外膜表面。该多克隆抗体对人 GRP75 氨基末端片段对应的肽具有特异性，已成功用于免疫沉淀、Western blotting , 和免疫组织化学检测人和小鼠细胞中的 GRP75。它识别前体和成熟 GRP75，应该有助于研究 GRP75 的陪伴和应激反应功能。它不会与 HSP70 家族的其他成员发生交叉反应。
参考资料：1． Kim 等人，J. Immunol。 154:1614-1623, 1995.2。 Wadhwa, Kaul 和 Mitsui, Y. Cell Struct。函数, 19:1-10, 1994.3. Webster 等，DNA 和细胞生物学。 13:1213-1220, 1994.4。 Mizzen 等人，J. Biol。化学。 264:20664-20675, 1989Product DocumentsSpec Sheet GR02.pdfRelated ProductsProductCAT.#SizePriceAnti-Human CYP450 3A4, Rabbit PolyclonalPA320.1 mL$315.00CypExpress 2D6, 1.0 GramCE2D6.11.0 Gram$500.00抗髓过氧化物酶, 人中性粒细胞，兔 IgG 组分 GP501.0 ml$495.00抗人 VLDL 受体单克隆抗体 RA321.0 mg$615.00CUPRACFood and Beverage Antioxidant AssayFS021 Kit$395.00返回页首抗 GRP 75 C 端 $290.00SKUGR03产品概述一种 75 kDa 葡萄糖调节蛋白 (GRP75) 是一种线粒体基质蛋白，通常被认为是热休克蛋白 70 (HSP70) 类蛋白质的成员。 GRP75 在低葡萄糖和其他营养和环境应激条件下被诱导。已在免疫系统（PBP74；CSA）、肝脏（mtHSP70）和细胞系中鉴定出密切相关的蛋白质。 GRP75 参与线粒体中蛋白质易位、折叠和功能的各种伴侣功能。它还似乎参与抗原识别、细胞增殖和衰老。虽然主要定位于线粒体，但相关形式可能存在于胞质溶胶或细胞外膜表面。该多克隆抗体对人 GRP75 羧基末端片段对应的肽具有特异性，已成功用于免疫沉淀、Western blotting , 和免疫组织化学l 检测人体和啮齿动物组织中的GRP75。该抗体对成熟的 GRP75 具有选择性，不会与其他 HSP70 成员发生交叉反应。
参考资料：1． Kim 等人，J. Immunol。 154:1614-1623, 1995.2。 Wadhwa, Kaul 和 Mitsui, Y. Cell Struct。函数, 19:1-10, 1994.3. Webster 等，DNA 和细胞生物学。 13:1213-1220, 1994.4。 Mizzen 等人，J. Biol。化学。 264:20664-20675, 1989Product DocumentsSpec Sheet GR03_0.pdf相关产品ProductCAT.#SizePriceHuman PAI-1（糖基化-潜伏形式）PL770.5 mg$1,145.00CypExpress 1A1, 10.0 克CE1A1.1010.0 克$3,760.00An ti-髓过氧化物酶，人中性粒细胞，兔 IgG 组分 GP501。 0 毫升 495.00 美元人类因子 XCF350.5 毫克 495.00 美元 GST A1-1，重组人 GS600.1 毫克 460.00 美元 Dopamine in the Dorsal Hippocampus Impairs the Late Consolidation of Cocaine-Associated Memory AbstractCocaine is thought to be addictive because it elevates dopamine levels in the striatum, reinforcing drug-seeking habits. Cocaine also elevates dopamine levels in the hippocampus, a structure involved in contextual conditioning as well as in reward function. Hippocampal dopamine promotes the late phase of consolidation of an aversive step-down avoidance memory. Here, we examined the role of hippocampal dopamine function in the persistence of the conditioned increase in preference for a cocaine-associated compartment. Blocking dorsal hippocampal D1-type receptors (D1Rs) but not D2-type receptors (D2Rs) 12鈥塰 after a single training trial extended persistence of the normally short-lived memory; conversely, a general and a specific phospholipase C-coupled D1R agonist (but not a D2R or adenylyl cyclase-coupled D1R agonist) decreased the persistence of the normally long-lived memory established by three-trial training. These effects of D1 agents were opposite to those previously established in a step-down avoidance task, and were here also found to be opposite to those in a lithium chloride-conditioned avoidance task. After returning to normal following cocaine injection, dopamine levels in the dorsal hippocampus were found elevated again at the time when dopamine antagonists and agonists were effective: between 13 and 17鈥塰 after cocaine injection. These findings confirm that, long after the making of a cocaine鈥損lace association, hippocampal activity modulates memory consolidation for that association via a dopamine-dependent mechanism. They suggest a dynamic role for dorsal hippocampal dopamine in this late-phase memory consolidation and, unexpectedly, differential roles for late consolidation of memories for places that induce approach or withdrawal because of a drug association. INTRODUCTIONThe addicted brain differs from the non-addicted brain as a function of two forms of neuroplasticity that are important for addiction theory: neuroadaptations resulting from the pharmacological impact of the drug on the brain and synaptic changes that reflect the memory trace for the drug experience (Berke and Hyman, 2000; Hyman et al, 2006). Neuroadaptations that are caused by repeated passive exposure to the drug itself have been widely studied (Nestler, 1992; L眉scher and Malenka, 2011), but there are further changes in brain function that reflect the memory of drug experience. These further brain changes are seen in animals that self-administer the drug, but are absent in animals that have passively received 鈥榶oked鈥?injections of the same drug at the same dose and temporal pattern (Wang et al, 2005; You et al, 2007, 2008; Chen et al, 2008; Caill茅 et al, 2009). Here, we were concerned with brain changes involved in the consolidation of the memory for drug鈥揺nvironment association established by Pavlovian conditioning.Much of the interest in the neuroplasticity associated with cocaine addiction has been focused on the ventral tegmental dopamine neurons (Bonci and Malenka, 1999; Saal et al, 2003) and their projection targets in the nucleus accumbens (Nestler, 1992; Hyman et al, 2006; Kalivas, 2009). Self-administered cocaine elevates dopamine levels there (Pettit et al, 1990; Wise et al, 1995), and the rewarding effects of self-administered cocaine are attenuated or blocked by lesions or infusion of dopamine antagonists into this area. Cocaine (Ikemoto, 2003) and dopamine agonists (Ikemoto and Goeders, 1998) are reinforcing when injected into the medial portions of the ventral striatum. However, while the striatum is thought to have the major role in the establishment of instrumental response habits (Everitt and Robbins, 2005), rats not only learn to self-administer addictive drugs but they also learn to approach and spend more time in the places where they have experienced addictive drugs in the past. Such learning is likely to implicate the hippocampal circuitry involved in spatial and contextual learning (O\'Keefe and Nadel, 1978; Phillips and LeDoux, 1994). One such circuit may involve the functional loops linking the ventral tegmental area (VTA) and the hippocampus (Lisman and Grace, 2005; Luo et al, 2011). Recent findings point to the dorsal hippocampus, and particularly to its dopaminergic inputs, as important for the persistence of memory storage in behaving rats (Rossato et al, 2009). This modulation is mediated by dopamine (acting at D1-type receptors (D1Rs)) and brain-derived neurotrophic factor (BDNF) 12鈥塰 after training (but not 0, 9, or 24鈥塰 after training), and is selective for the maintenance, but not for the formation, of fear-associated long-term memories (LTMs) (Bekinschtein et al, 2007; Rossato et al, 2009). However, the mechanisms by which a drug-related memory becomes persistent over time remain to be elucidated. In the present study, we explore the role of dorsal hippocampal dopamine in modulating the persistence of memories for the association between rewarding cocaine or aversive lithium chloride (LiCl) and the experimental chamber in which the effects of the drugs were experienced.MATERIALS AND METHODSSee Supplementary Materials and methods鈥?for further details.AnimalsA total of 949 male Sprague鈥揇awley rats (Facultad de Ciencias Veterinarias, Argentina; and Charles River, Raleigh, NC) were used. Groups of five rats each (weighting around 200鈥塯 upon arrival at the laboratory) were housed in a vivarium maintained on a reversed 12-h light鈥揹ark cycle (lights off at 0630 hours) at a constant temperature of 23鈥壜癈. Animal care was in strict accordance with institutional and international standards (National Research Council, 2011; NIDA Animal Care and Use Committee).DrugsCocaine hydrochloride (Laboratorios Verardo y Cia., Buenos Aires, Argentina or NIDA intramural pharmacy, Baltimore, MD), SCH 23390 hydrochloride, quinpirole hydrochloride (Sigma-Aldrich, St Louis, MO), eticlopride hydrochloride (Tocris Bioscience, Bristol, UK), LiCl (Laboratorio Cicarelli, San Lorenzo, Argentina) were all dissolved in sterile 0.9% physiological saline. SKF 38393 hydrochloride, SKF 83959 hydrobromide (Sigma-Aldrich), and SKF 83822 hydrobromide (Tocris Bioscience) were dissolved in sterile 0.9% physiological saline supplemented with DMSO (10%, final concentration; Laboratorio Ernesto van Rossum y Cia., Buenos Aires, Argentina). All the antibodies, except for anti-phospho-ERK1/2 and anti-ERK1/2 (Cell Signaling Technology, Beverly, MA), were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).Surgical and Intracerebral Infusion ProceduresEach rat was anesthetized with a mix of ketamine (85鈥塵g/kg) and xylazine (10鈥塵g/kg), and placed on a stereotaxic frame. The skull was exposed and leveled (flat skull, lambda, and bregma at the same elevation). Either 22-G guide cannulae for intracerebral infusions or CMA 11 microdialysis guide cannulae (CMA, Helsingborg, Sweden) were bilaterally implanted, aimed at the dorsal hippocampus (CA1 region). The stereotaxic coordinates used were as follows: AP: 鈭?.9; ML: 卤3.0; DV: 鈭?.0 (Paxinos and Watson, 2004). Animals were allowed 5鈥? days of recovery before any experimental manipulation.For intracerebral infusions, 30-G needles connected to Hamilton syringes were used. The infusions were always bilateral, with volume infusions of 1鈥壩糽 per side. The needle was left in place for an additional minute after infusion to allow diffusion and to prevent reflux. The vehicle used in each case corresponds to the solution in which the respective drug was dissolved (see Drugs section above). The doses used for each compound were as follows: 1鈥壩糶/渭l for quinpirole and SKF 83822, 1.5鈥壩糶/渭l for SCH 23390 and eticlopride, 10鈥壩糶/渭l for SKF 83959, and 12.5鈥壩糶/渭l for SKF 38393. At the end of each experiment, cannula placement was verified by infusion of 1鈥壩糽 of methylene blue and histological analysis. Only the behavioral data of animals with a correct placement of cannulae were included in the study.In Vivo MicrodialysisAnimals were connected to the microdialysis systems the evening previous to the microdialysis experiment. The blocker of each guide cannula was removed and the microdialysis probe (CMA 11, 1鈥塵m membrane length; CMA) was inserted and secured in place. Probes were connected to the microdialysis pump (CMA/100; CMA) with FEP tubing through a two-channel swivel and were perfused overnight with an artificial cerebrospinal fluid, at a flow rate of 0.3鈥壩糽/min. Next morning, the flow rate was increased to 0.6鈥壩糽/min, and after 1鈥塰 of equilibration, a series of four 30-min baseline samples were collected. Then, animals were intraperitoneally injected with either saline or cocaine (20鈥塵g/kg) and confined to the corresponding side of a three-compartment apparatus (see Behavioral training and testing section below) during 30鈥塵in. They were returned to their home cage afterwards. Microdialysis samples were collected every hour during a 24-h period. Acetic acid (20鈥塵M, 6鈥壩糽) was added to the vials where samples were collected to avoid dopamine degradation.Biochemical Analysis of Microdialysis SamplesDialysate dopamine concentrations were analyzed using high-performance liquid chromatography (HPLC) with electrochemical detection. Aliquots from dialysate samples (15鈥壩糽) were injected into an Eicom HTEC-500 HPLC system with an integrated amperometric detector, consisting a PP-ODS HPLC column (30 脳 4.6鈥塵m2, inner diameter, C-18, 2鈥壩糾 particulate silica gel) and an HTEC-500 pump (Eicom, San Diego, CA). The mobile phase consisted of 100鈥塵M NaH2PO4, 1.3鈥塵M EDTA, 2.0鈥塵M decane-1-sulfonate, and 1% MeOH, pH 6.0, resulting in a retention time of 2.2鈥?.5鈥塵in at a pump rate of 0.5鈥塵l/min. Applied potential was set at +450鈥塵V vs Ag/AgCl.Western Blot AssaysThree different conditions were analyzed: (1) biochemical profiles of BDNF, p-TrkB and p-ERK1/2 12鈥塰 after cocaine (or saline in the case of controls) injection in one-trial training; (2) biochemical profiles of BDNF and p-TrkB 12鈥塰 after the last injection of cocaine (or saline in the case of controls) in three-trial training; and (3) biochemical profiles of BDNF and p-ERK1/2 after the infusion of the D1R antagonist SCH 23390 in the dorsal hippocampus. The dopaminergic antagonist was infused 11.5鈥塰 after the injection of cocaine in one-trial training. Thirty minutes later, animals were killed; the dorsal hippocampi were dissected and rapidly homogenized in ice-chilled buffer. Samples of homogenates (10鈥壩糶 of protein) were subjected to SDS-PAGE (10 or 15% gels) under reducing conditions. Proteins were electrotransferred onto PVDF membranes for 2鈥塰 at 100鈥塚 at 4鈥壜癈 for BDNF analysis. For ERK1/2 and p-TrkB analysis, proteins were transferred onto PVDF membranes overnight at 40鈥塚 at 4鈥壜癈. Immunoblots were performed by incubating membranes with antibodies anti-BDNF (1:1000, sc-546), anti-actin (1:10鈥?00, sc-1615), anti-phospho-ERK1/2 (1:4000, no. 9101s), anti-ERK1/2 (1:3000, no. 9102), anti-phospho-TrkB (1:2000, sc-135645), and anti-TrkB (1:1000, sc-8316). The blots were scanned in a Storm 845 PhosphorImager (GE Healthcare Life Sciences, Little Chalfont, UK), and quantifications were carried out with ImageQuant software (GE Healthcare Life Sciences).Behavioral Training and TestingConditioningPlace conditioning was carried out in three-compartment chambers; the center compartment was a short connecting passageway between the conditioning chambers in which the animals were placed after drug injections. One conditioning compartment had black walls (white squares pattern, grid floor) and the other had white walls (black lines pattern, perforated floor); the connecting passage was gray (no pattern, smooth floor). The experiments consisted of three phases: a pretest phase in which the animals were allowed to explore the entire apparatus for 15鈥塵in, a conditioning phase in which they were restricted to one side of the chamber after saline intraperitoneal injections and, on alternate days, the other side of the chamber after saline, cocaine, or LiCl injections, and a test phase in which they were allowed to explore the open apparatus again in a drug-free state. During the pretest phase, if an animal failed to spend at least 90鈥塻 in each compartment, it was excluded from the study. Five out of 954 animals were excluded for this reason.In the one-trial version of the cocaine conditioning paradigm, the animals were given an intraperitoneal saline injection before placement in the initially preferred dark chamber for 30鈥塵in on the first conditioning day and were given an intraperitoneal cocaine injection before placement in the initially non-preferred white chamber for 30鈥塵in on the following day. Saline intraperitoneal injections were given in both compartments for control groups. The one-trial version was used to establish a weak memory for the cocaine-chamber association in experiments designed to assess the effects of treatments designed to increase memory persistence; the procedure was repeated two times more in a three-trial version to establish a stronger memory for the same association in experiments designed to assess treatments that might decrease memory persistence. Cocaine doses of 10, 15, or 20鈥塵g/kg were used for the dose鈥搑esponse experiment, and 20鈥塵g/kg was used for the remaining experiments. An increase in time spent in the cocaine-associated chamber after conditioning was taken as a reflection of the known rewarding properties of cocaine at this dose (Busse and Riley, 2004; dela Cruz et al, 2009; Crooks et al, 2010).In the place conditioning studies with LiCl, the one-trial version was used. On the first day, the animals were given intraperitoneal saline and placed in the initially non-preferred white chamber for 60鈥塵in; on the next day, the animals were given intraperitoneal saline or LiCl (150鈥塵g/kg) and placed in the initially preferred black compartment for 60鈥塵in. In each case, the animals were given open access to the entire apparatus on the day following the last conditioning day; this phase was identical to the pretest: animals in a drug-free state were left to explore the entire apparatus during 15鈥塵in and the time spent in each compartment was recorded. A decrease in time spent in the LiCl-associated chamber after conditioning was taken as a reflection of the known aversive properties of LiCl at this dose (Tenk et al, 2005).Supplementary Table 1 shows the mean pre- and postconditioning times spent in the compartment associated with cocaine or LiCl for each of the main results in this study.In experiments where intracerebral infusions were performed, the infusion time was always 12鈥塰 after conditioning. Animals were infused and returned immediately to their home cages. They were never exposed to the testing apparatus during or after the infusion procedure. When a three-trial training protocol was used, the dopaminergic drugs were infused 12鈥塰 after each conditioning session with either cocaine or LiCl.Statistical AnalysesData were presented as a score in s: time spent in the drug-associated compartment during the test minus time spent in the to-be drug-associated compartment during the pretest. Results are presented as mean卤SEM. For behavioral and biochemical data, results were analyzed using a Student鈥檚 t-test in the case where two groups were to be compared and using a multifactorial analysis of variance (MANOVA) with cocaine/LiCl (saline vs cocaine/LiCl), dose (10, 15, or 20鈥塵g/kg), place (home cage vs conditioning apparatus), time of testing (24鈥塰, and 7 or 14 days), and challenge (vehicle vs dopaminergic agents) as between-subject factors, in the case where more than two groups were to be compared. For significant overall interactions, further analyses of partial interactions were carried out. Post hoc analyses were performed with Newman鈥揔euls test when the initial p-value was significant. A result was considered significant if p 0.05. All data were analyzed using Statistica software (Statsoft, Paris, France).RESULTSWe first established conditioning parameters that would result in long-lasting (7 days or more) or short-lasting (24鈥塰 but not 7 days) increases in time spent in the cocaine-associated chamber using independent animal groups. Increased times in the cocaine-associated chamber were seen in rats trained with a single injection (20鈥塵g/kg) and tested 1 day but not 7 or 14 days after the conditioning trial (Figure 1a, main effect of cocaine: F(1,58)=31.94, p 0.001; main effect of dose: F(2,58)=0.85, p=0.43, NS; cocaine 脳 dose: F(2,58)=3.70, p 0.05; Figure 1b, main effect of cocaine: F(1,44)=2.05, p=0.16, NS; main 脳 effect of time: F(2,44)=0.40, p=0.67, NS; cocaine 脳 time: F(2,44)=4.24, p 0.05). The reversed order of injections of cocaine and saline gave similar results (Supplementary Figure S1a; p 0.05). Rats given two cocaine training days also showed an increase in time spent in the cocaine-associated chamber when tested (1 day but not 7 days) after conditioning (Supplementary Figure S1b; p=0.18). Independent groups of rats given three cocaine鈥揷hamber pairings showed increased time in the cocaine-associated chamber when tested 7, 14, or 21 days after training (Figure 1c, main effect of cocaine: F(1,62)=27.74, p 0.001; main effect of time: F(3,62)=0.83, p=0.48, NS; cocaine 脳 time: F(3,62)=4.43, p 0.05). We subsequently used the single pairing condition to establish weakly persistent memory and the three-pairing condition to establish stronger, more persistent memory for the cocaine鈥揷hamber association.Figure 1Temporal duration and dopaminergic modulation of the persistence of a short-lasting cocaine-associated memory. (a) Three doses of cocaine were tested. The dose of 20鈥塵g/kg induced a significant retention of the memory for cocaine鈥損lace association when tested 24鈥塰 after a one-trial training (n=5鈥?1 per group). Therefore, this dose was used in all the remaining approach experiments. (b) A weak (one-trial training) memory for the cocaine鈥損lace association was retained for 24鈥塰 but not for 7 or 14 days (n=7鈥?1 per group). (c) A strong (three-trial training) memory for the cocaine鈥損lace association was retained for at least 21 days (n=8鈥?0 per group). Asterisks indicate significant differences between cocaine-injected animals and its corresponding saline controls (Newman鈥揔euls, *p 0.05; **p 0.01). (d鈥揼) Effects of D1-type dopamine agonist (SKF 38393) or antagonist (SCH 23390) injections鈥攇iven 12鈥塰 after the training trial鈥攐n the persistence of the memory for cocaine鈥損lace associations established by one-trial training. (d) The D1-type agonist had no effect on the 24鈥塰 memory for cocaine鈥損lace associations established by one-trial training (n=8鈥?0 per group). (e) The D1-type agonist had no effect on the persistence of the memory for cocaine鈥損lace associations when tested at 7 days after one-trial training (n=10鈥?2 per group). (f) The D1-type antagonist had no effect on the 24鈥塰 memory for cocaine鈥損lace associations established by one-trial training (n=16鈥?7 per group). (g) The D1-type antagonist prolonged the duration of the memory for cocaine鈥損lace associations after one-trial training (n=11鈥?4 per group). Post hoc analyses demonstrated that the group conditioned with cocaine, infused with SCH 23390 12鈥塰 later, and tested at 7 days showed a higher score when compared with the other groups (Newman鈥揔euls, **p 0.01). Asterisks upon bracket indicate a significant difference between saline- and cocaine-injected animals, collapsed across intracerebral infusion treatments (Newman鈥揔euls, *p 0.05; **p 0.01). Coc, cocaine; Sal, saline; Veh, vehicle. PowerPoint slide Full size imageTo determine the effects of dorsal hippocampal dopamine receptor activation on the late post-training consolidation of the memory for the cocaine鈥揷hamber association, we microinjected dopamine receptor agonists or antagonists into the dorsal hippocampus 12鈥塰 after one- or three-trial training and tested for retention 24鈥塰 or 7 days later (see Supplementary Figure S2 to check for the location and extension of the infusions). Rats tested 24鈥塰 after one-trial training showed normal retention following bilateral hippocampal microinjections of either the D1R agonist SKF 38393 or the D1R antagonist SCH 23390 (Figure 1d, main effect of cocaine: F(1,31)=16.54, p 0.001; main effect of challenge: F(1,31)=2.12, p=0.16, NS; cocaine 脳 challenge: F(1,31)=0.04, p=0.84, NS; Figure 1f, main effect of cocaine: F(1,62)=23.87, p 0.001; main effect of challenge: F(1,62)=3.69, p=0.06, NS; cocaine 脳 challenge: F(1,62)=0.04, p=0.84, NS). The dopamine D1R agonist failed to modify the retention of the cocaine鈥揷hamber association when tested 7 days after training (Figure 1e, main effect of cocaine: F(1,40)=0.34, p=0.57, NS; main effect of challenge: F(1,40)=0.009, p=0.93, NS; cocaine 脳 challenge: F(1,40)=0.05, p=0.82, NS). However, treatment with the D1R antagonist unexpectedly extended retention of the association to 7 days after one-trial training (Figure 1g, main effect of cocaine: F(1,47)=4.64, p 0.05; main effect of challenge: F(1,47)=4.33, p 0.05; cocaine 脳 challenge: F(1,47)=7.60, p 0.01).We next determined the effects of the same treatments on the persistence of the stronger memory established with the three-trial training paradigm. As in the case of the short-lasting cocaine-associated memory, neither the D1R antagonist nor the D1R agonist, given 12鈥塰 after training, affected retention as tested 24鈥塰 after training (Figure 2a, main effect of cocaine: F(1,35)=28.69, p 0.001; main effect of challenge: F(1,35)=0.24, p=0.63, NS; cocaine 脳 challenge: F(1,35)=0.73, p=0.40, NS; Figure 2c, main effect of cocaine: F(1,31)=28.18, p 0.001; main effect of challenge: F(1,31)=0.11, p=0.74, NS; cocaine 脳 challenge: F(1,31)=0.48, p=0.49, NS). The D1R antagonist also failed to affect retention as tested at 7 days (Figure 2d, main effect of cocaine: F(1,53)=19.96, p 0.001; main effect of challenge: F(1,53)=0.03, p=0.85, NS; cocaine 脳 challenge: F(1,53)=0.04, p=0.85, NS). Conversely, when the D1R agonist was administered during the three training trials, the persistence of a normally long-lasting cocaine-associated memory was abolished (Figure 2b, main effect of cocaine: F(1,31)=7.44, p 0.05; main effect of challenge: F(1,31)=5.65, p 0.05; cocaine 脳 challenge: F(1,31)=4.25, p 0.05). Interestingly, the D1R agonist was ineffective in three cases where the cannula placement was asymmetrical and injections did not reach the dorsal hippocampus or reached it on one side only (Supplementary Figure S3).Figure 2Dopaminergic modulation of the persistence of a long-lasting cocaine-associated memory. Effects of D1-type dopamine agonist (SKF 38393) or antagonist (SCH 23390) injections鈥攇iven 12鈥塰 after the training trial鈥攐n the persistence of the memory for cocaine鈥損lace associations established by 3-trial training. (a) The D1-type agonist (SKF 38393) had no effect on the 24鈥塰 memory for cocaine鈥損lace associations established by three-trial training (n=9鈥?0 per group). (b) Treatment with the D1-type agonist (SKF 38393) attenuated the 7-day persistence of the memory for cocaine鈥損lace associations established by three-trial training (n=7鈥?0 per group). (c) The D1-type antagonist (SCH 23390) had no effect on the 24鈥塰 memory for cocaine鈥損lace associations established by three-trial training (n=8鈥?0 per group). (d) The D1-type antagonist (SCH 23390) had no effect on the persistence of the memory for cocaine鈥損lace associations when tested at 7 days after three-trial training (n=13鈥?5 per group). Post hoc analyses demonstrated that the group conditioned with cocaine, infused with SKF 38393 12鈥塰 later, and tested at 7 days showed a higher score when compared with the other groups (Newman鈥揔euls, **p 0.01). Asterisks upon bracket indicate a significant difference between saline and cocaine-injected animals, collapsed across intracerebral infusion treatments (Newman鈥揔euls, **p 0.01; ***p 0.001). Coc, cocaine; Sal, saline; Veh, vehicle. PowerPoint slide Full size imageNeither the D2-type dopamine receptor agonist quinpirole (1鈥壩糶 per side; main effect of cocaine: F(1,41)=2.23, p=0.14, NS; main effect of challenge: F(1,41)=0.0004, p=0.98, NS; cocaine 脳 challenge: F(1,41)=0.06, p=0.80, NS) nor the D2-type dopamine receptor antagonist eticlopride (1.5鈥壩糶 per side; main effect of cocaine: F(1,31)=1.03, p=0.32, NS; main effect of challenge: F(1,31)=0.04, p=0.83, NS; cocaine 脳 challenge: F(1,31)=0.13, p=0.72, NS) affected the persistence of the weak cocaine-associated memory (Supplementary Figure S4).To gain better insight on the molecular mechanisms underlying the persistence of cocaine-associated memories, we next used agonists for D1Rs that are selectively coupled to different intracellular messenger cascades (Undieh, 2010). Whereas the adenylyl cyclase (AC)-selective agonist SKF 83822 given 12鈥塰 after three-trial training had no effect on retention tested 7 days after training (Figure 3a, main effect of cocaine: F(1,35)=12.16, p 0.01; main effect of challenge: F(1,35)=1.31, p=0.26, NS; cocaine 脳 challenge: F(1,35)=0.07, p=0.79, NS), the phospholipase C (PLC)-coupled agonist SKF 83959 significantly decreased retention at the same time-point (Figure 3b, main effect of cocaine: F(1,26)=12.40, p 0.01; main effect of challenge: F(1,26)=8.33, p 0.01; cocaine 脳 challenge: F(1,26)=5.72, p 0.05).Figure 3Persistence of cocaine-related memories is regulated by dopaminergic receptors coupled to phospholipase C (PLC) cascade and is not mediated by brain-derived neurotrophic factor (BDNF). (a) Delayed (12鈥塰 after conditioning trial) infusion of the adenylyl cyclase (AC)-selective D1-type agonist SKF 83822 had no effect on the 7-day retention of the memory for cocaine鈥損lace associations established by three-trial training (n=9鈥?0 per group). (b) Delayed (12鈥塰 after conditioning trial) infusion of the PLC-selective D1-type agonist SKF 83959 significantly attenuated 7-day retention of the memory for cocaine鈥損lace associations established by three-trial training (n=7鈥? per group). Post hoc analyses demonstrated that the group conditioned with cocaine, infused with SKF 83959 12鈥塰 later, and tested at 7 days showed a higher score when compared with the other groups (Newman鈥揔euls, *p 0.05). Asterisks upon bracket indicate a significant difference between saline- and cocaine-injected animals, collapsed across intracerebral infusion treatments (Newman鈥揔euls, **p 0.01). (c鈥揺) Dorsal hippocampal levels of BDNF of animals administered either saline or 20鈥塵g/kg cocaine. Animals were injected and returned to their home cages (HC groups) or were conditioned as described (C groups). In all the figures, control group is represented by animals injected with saline and left undisturbed in their home cages (Sal/HC groups). (c) Levels of dorsal hippocampal BDNF measured at 12鈥塰 after one-trial training. BDNF levels did not vary across treatments (n=5 per group). (d) Levels of dorsal hippocampal BDNF measured at 12鈥塰 after three-trial training. BDNF levels did not vary across treatments (n=3鈥? per group). (e) SCH 23390 administration did not modify the dorsal hippocampal levels of BDNF measured at 12鈥塰 after one-trial training. Animals were conditioned with either saline or cocaine; 11.5鈥塰 later they were infused with SCH 23390 or its vehicle and were killed 30鈥塵in later (n=15 per group). Coc, cocaine; Sal, saline; Veh, vehicle. PowerPoint slide Full size imageTo further investigate the hypothesis that persistent cocaine-associated memories depend on D1Rs not coupled to AC, we measured the expression of BDNF, a downstream component of D1Rs/AC/PKA/BDNF/ERK pathway known to be elevated in the dorsal hippocampus 12鈥塰 after training in long-lasting fear memories (Bekinschtein et al, 2007; Rossato et al, 2009). When BDNF levels were measured 12鈥塰 after training, no differences were observed either in animals subjected to the one-trial (Figure 3c, main effect of cocaine: F(1,16)=0.88, p=0.36, NS; main effect of place: F(1,16)=0.24, p=0.63, NS; cocaine 脳 place: F(1,16)=0.26, p=0.61, NS) or to the three-trial (Figure 3d, main effect of cocaine: F(1,10)=0.0003, p=0.99, NS; main effect of place: F(1,10)=2.15, p=0.17, NS; cocaine 脳 place: F(1,10)=0.89, p=0.37, NS) training. Furthermore, BDNF levels were not affected by dorsal hippocampal injections of the D1R antagonist SCH 23390, a treatment that improved the persistence of a weak cocaine-related memory (Figure 3e, main effect of group: F(2,42)=0.99, p=0.38, NS). In addition, no alterations were observed in the phosphorylation state of TrkB, or in the activation of ERK1/2, which are downstream steps of BDNF-induced memory persistence in the case of fear memories (Supplementary Figure S5).It was not expected that dopamine agonists would inhibit the late-phase of cocaine-associated memory consolidation because dopamine is known to be necessary for promoting the late-phase of aversive memory consolidation in a step-down avoidance task (Rossato et al, 2009). To determine the role of dorsal hippocampal dopamine in an avoidance context, we substituted the rewarding agent cocaine by an aversive agent. Here we used LiCl, an aversive agent known to be capable of establishing taste and place avoidance (Nachman, 1963; Tenk et al, 2005) as our unconditioned stimulus. In our place-conditioning paradigm, it had the opposite effect of cocaine; where rats increased the time they spent in the cocaine-associated chamber, other independent group of rats decreased the time spent in the LiCl-associated chamber. This effect was seen 24鈥塰 but not 7 days after training (Supplementary Figure S6; p 0.05). Bilateral dorsal hippocampal infusions of the D1R agonist SKF 38393 given 12鈥塰 after training facilitated the persistence of the LiCl-associated memory, which remained strong 7 days after training (Figure 4, main effect of LiCl: F(1,30)=5.98, p 0.05; main effect of challenge: F(1,30)=13.81, p 0.001; LiCl 脳 challenge: F(1,30)=4.55, p 0.05). Thus, late post-training activation of hippocampal D1Rs had opposite effects on the persistence of the memory for learned cocaine鈥揷ontext and LiCl鈥揷ontext associations, increasing the persistence of the memory for the association between LiCl and the place where it was given and decreasing the persistence of the memory for the association between cocaine and the place where it was given.Figure 4Effects of D1-type dopamine agonist (SKF 38393) on the persistence of the memory for one-trial LiCl鈥損lace associations. Infusion of the D1-type agonist (SKF 38393) enhanced the retention of the 7-day memory for the place associations induced by LiCl (n=7鈥?). Asterisk indicates significant post hoc comparisons (Newman鈥揔euls, *p 0.05). LiCl, lithium chloride; Sal, saline; Veh, vehicle. PowerPoint slide Full size imageThe dopamine-dependent potentiation of late-phase memory consolidation through PLC-coupled D1Rs might require only normal baseline levels of hippocampal dopamine. To determine if there are normal or fluctuating levels of dopamine at the time when dopamine inhibits late-phase memory consolidation for cocaine鈥損lace associations, we used microdialysis to monitor dorsal hippocampal dopamine levels over the 24鈥塰 after cocaine treatment. Here, we used the one-trial paradigm. After a large but variable (and thus statistically unreliable) surge following the training injection of cocaine, dopamine levels returned to a stable baseline within about 5鈥塰, but increased again for a period of 4鈥塰 starting 13鈥塰 after the training session (Figure 5). The increase began 3鈥塰 after the onset of the light phase of the light鈥揹ark cycle, at a time when striatal dopamine levels normally become depressed (Casta帽eda et al, 2004); thus, it was not obviously time-locked to the light change. The cocaine-injected animals showed a reliable increase in approach to the cocaine-associated chamber when tested 4鈥塰 after the completion of the microdialysis experiments (Supplementary Figure S7; p 0.05).Figure 5Dopamine (DA) fluctuations in microdialysis samples from the dorsal hippocampus of animals following one-trial training for cocaine鈥損lace associations. Cocaine or saline was given at the arrow after the baseline period (B) and hourly samples were taken for the next 24鈥塰. The inset shows the results of four baseline samples pooled together. There were no significant differences in baseline between the two groups (n=7 per group; Student鈥檚 t-test, t(12)=鈭?.31; p=0.76). There were no significant differences in DA measurements between groups during the first 12-h period of the experiment (main effect of cocaine: F(1,12)=1.72; p=0.21, NS; main effect of time: F(11,132)=1.30; p=0.23, NS; cocaine 脳 time interaction: F(11,132)=0.69; p=0.74, NS). However, during the second 12-h period, there was a significant main effect of time, F(11,132)=2.04; p=0.03 and a significant cocaine 脳 time interaction, F(11,132)=4.17; p=0.00003. Asterisks indicate significant post hoc comparisons (Newman鈥揔euls, **p 0.01; ***p 0.001). The horizontal bar in the bottom of the figure indicates the dark鈥搇ight cycle (black: lights out). PowerPoint slide Full size imageDISCUSSIONThe present findings have two important implications. First, we report for the first time a role for dopamine in the dorsal hippocampus on the long-term storage for a cocaine-associated memory, an influence on memory storage that develops long after the elevation in dopamine levels caused by cocaine itself. Second, we report opposite effects of hippocampal dopamine actions on the memory for the association of neutral environmental place cues with the rewarding drug cocaine on the one hand or the memory for the association of the environmental place cues with the aversive drug LiCl on the other; stimulating dorsal hippocampal D1-type dopamine receptors decreased the persistence of the memory for a cocaine鈥損lace association, but increased the persistence of LiCl鈥損lace memory.The persistence of a weak cocaine-conditioned memory was enhanced by blocking dorsal hippocampus D1Rs 12鈥塰 after conditioning; it was attenuated by stimulating these receptors. The effects were not due to enhancing or blocking the impact of the acute extracellular dopamine elevation resulting from cocaine鈥檚 blockade of the dopamine transporter, as dopamine levels had returned to normal within 5鈥?鈥塰 of cocaine injection. However, there was a 4-h surge in extracellular dopamine concentration that began about 13鈥塰 after the cocaine injection, and there was neither a pharmacological treatment nor a change in environmental stimulus conditions at that time. This surge in dopamine levels was presumably caused by an increase in dopamine cells firing in the VTA, and the cause of such an increase is a matter for future investigation. That microinjection of D1R agonists attenuated retention of the cocaine-associated memory suggests that the increase in dopamine 13鈥塰 after cocaine place conditioning is itself a limiting factor in the persistence of cocaine-associated memories.This observation is surprising in that it is inconsistent with what is known of the role of dorsal hippocampal dopamine in late-phase consolidation of instrumental avoidance memories (Rossato et al, 2009). Indeed, the effects of dopamine receptor manipulations in our task were opposite to those previously reported for a step-down avoidance task; in the avoidance task, dopamine agonists administered to the dorsal hippocampus 12鈥塰 (but not 0 or 9鈥塰) after conditioning promoted late-stage memory consolidation, whereas dopamine antagonists restricted it (Rossato et al, 2009). To be sure that our finding was not unique to our place-conditioning paradigm, we confirmed that a D1R agonist facilitates late-phase avoidance memory persistence in a paradigm similar to ours, but using an aversive rather than a rewarding stimulus. Our LiCl conditioning task involved the same equipment and protocol as was used for cocaine conditioning, but using the aversive agent LiCl rather than the appetitive agent cocaine, as the unconditioned stimulus. Our results thus suggest opposite roles for dorsal hippocampal dopamine in the late phase of LTM storage of memories associated with rewarding and aversive stimuli.The obtained results suggest that the dopaminergic agents used involved actions at a given subtype of the D1-like dopamine receptors family. D1-like receptors are subdivided into D1 and D5 subtypes (Monsma et al, 1990; Sunahara et al, 1991), each of which is expressed in hippocampal neurons. The D5 subtype, presumably the one subtype coupled to PLC (Sahu et al, 2009; Undieh, 2010), is densely expressed in the hippocampus (Laurier et al, 1994) and has a 10-fold higher affinity for DA than does the D1 subtype (Sunahara et al, 1991). We found that SKF 83959 (thought to act specifically at the D5 receptor because it induces the selective activation of PLC intracellular cascade; Undie et al, 1994) had the inhibitory effect we saw with the more generic agonist, whereas SKF 83822, which induces activation of the AC intracellular cascade (O鈥橲ullivan et al, 2004), failed to do so. These data suggest that the D5 subtype is important for modulating the persistence of contextual memories associated with the rewarding drug cocaine, whereas the D1 subtype is important for modulating the persistence of contextual memories associated with the aversive agent LiCl (present study), along with those associated with the aversive effects of painful footshock (Rossato et al, 2009). Consistent with this suggestion, the levels of BDNF and the activation of TrkB and ERK1/2, which are downstream effectors of D1Rs coupled to AC in controlling the persistence of an aversive memory (Rossato et al, 2009), were unchanged 12鈥塰 after cocaine-context conditioning.These and earlier studies involving step-down avoidance (Rossato et al, 2009) make it clear that dopamine-dependent events taking place in the dorsal hippocampus around 12鈥塰 after a learning experience can enhance or limit the persistence of the memory for that experience (Supplementary Figure S8). One possibility is that dopamine is enhancing or inhibiting the continuing process of long-term storage of the original memory for the learning event. Another possibility to consider is whether dopamine is enhancing or inhibiting the reconsolidation of a reactivated trace. Consistent with this latter view is that the dopamine system, at least, is clearly reactivated at about this time. Against that view, however, are several facts. First, there is no obvious source of reactivation of the initial memory; the animals were not exposed to the contextual stimuli associated with the learning experience until they were tested 24鈥塰 or more hours later. Second, the effects of dopamine manipulations were time-locked to the original training; when given in the same way with the same attendant handling and lab activity at earlier times, the dopamine manipulations are ineffective (Rossato et al, 2009). Also seemingly inconsistent with any hypothesis involving spontaneous reactivation and reconsolidation during subsequent sleep is the fact that D1R activation had opposite effects on the duration of memories for place associations with rewarding and aversive stimuli.The fact that the memory for a cocaine鈥損lace association is still labile 12鈥塰 after the event has potential implications for addiction therapy. 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J Neurosci 28: 9021鈥?029.CAS聽 Article聽Google Scholar聽 Download referencesAcknowledgementsWe gratefully acknowledge Dr Gustavo Murer and Verardo y C铆a. laboratory for their kind donation of quinpirole and cocaine hydrochloride, respectively. We thank Catherine Schweppe, Fernando Garagoli, Eduardo Gigante, and Carolina Gonz谩lez for excellent technical help. CP Kramar was supported by a doctoral fellowship from CONICET, Argentina. This work was supported by CONICET, UBA, Foncyt (Grants PICT 2010-1169 to Dr Medina and PICT 2011-1941 to Dr Barbano) and by the Intramural Research Program of the National Institute on Drug Abuse (NIDA/NIH). Authors contribution CPK and MFB carried out the behavioral experiments. MFB conducted the microdialysis experiments. VIC performed the biochemical analyses of the microdialysis samples. CPK carried out the western blot assays. JHM and MFB designed the study with CPK and RAW. MFB, RAW, and JHM wrote the manuscript with the help of CPK.Author informationAffiliationsInstituto de Biolog铆a Celular y Neurociencias (CONICET-UBA), Facultad de Medicina, UBA, Buenos Aires, ArgentinaCecilia P Kramar,聽Jorge H Medina聽 聽M Flavia BarbanoNational Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, Baltimore, MD, USAVladimir I Chefer聽 聽Roy A WiseDepartamento de Fisiolog铆a, Facultad de Medicina, UBA, Buenos Aires, ArgentinaJorge H MedinaAuthorsCecilia P KramarView author publicationsYou can also search for this author in PubMed聽Google ScholarVladimir I CheferView author publicationsYou can also search for this author in PubMed聽Google ScholarRoy A WiseView author publicationsYou can also search for this author in PubMed聽Google ScholarJorge H MedinaView author publicationsYou can also search for this author in PubMed聽Google ScholarM Flavia BarbanoView author publicationsYou can also search for this author in PubMed聽Google ScholarCorresponding authorCorrespondence to M Flavia Barbano.Additional informationSupplementary Information accompanies the paper on the Neuropsychopharmacology websiteSupplementary information Supplementary Information (DOC 7639 kb)PowerPoint slides PowerPoint slide for Fig. 1PowerPoint slide for Fig. 2PowerPoint slide for Fig. 3PowerPoint slide for Fig. 4PowerPoint slide for Fig. 5Rights and permissionsReprints and PermissionsAbout this articleCite this articleKramar, C., Chefer, V., Wise, R. et al. Dopamine in the Dorsal Hippocampus Impairs the Late Consolidation of Cocaine-Associated Memory. Neuropsychopharmacol 39, 1645鈥?653 (2014). https://doi.org/10.1038/npp.2014.11Download citationReceived: 28 September 2013Revised: 06 December 2013Accepted: 13 January 2014Published: 20 January 2014Issue Date: June 2014DOI: https://doi.org/10.1038/npp.2014.11Keywordsmemory persistencememory consolidationhippocampusdopaminecocaineconditioned behavior Michael Wolter, Thomas Lapointe, Brett Melanson, Nana Baidoo, Travis Francis, Boyer D. Winters Francesco Leri Psychopharmacology (2021) Kiana Nouri, Mahsa Anooshe, Saeideh Karimi-Haghighi, Zahra Mousavi Abbas Haghparast Neurochemical Research (2021) Fernando Castillo D铆az, Micaela A. Hernandez, Tomas Capell谩 Jorge H. 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