首页 > avivasysbio > avivasysbio/IRF3 Antibody (Phospho-Ser396) (OABF01188)/100 ul/OABF01188

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avivasysbio/IRF3 Antibody (Phospho-Ser396) (OABF01188)/100 ul/OABF01188

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To generate large amounts of a recombinant protein, many investigators have turned to baculovirus expression systems. Compared to bacterial expression systems, the posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles mammalian processes, and the yields of functional protein are often much greater.To generate large amounts of a recombinant protein, many investigators have turned to baculovirus expression systems. Compared to bacterial expression systems, the posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles mammalian processes, and the yields of functional protein are often much greater.BacPAK methodThe BacPAK System uses the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) to produce target proteins in insect cells. The target gene is inserted into a shuttle vector, which is cotransfected into insect host cells with the linearized BacPAK6 Viral DNA. The specially designed BacPAK6 Viral DNA forces recombination between the virus and transfer vector, resulting in high recombination efficiency. Following recombination, a few viral plaques are picked and purified, and the recombinant phenotype is verified. The newly isolated recombinant virus can then be amplified and used to infect insect cell cultures to produce large amounts of the desired protein.If you wish, you can use pBacPAK8-GUS (sold as part of the BacPAK Baculovirus Expression System) as a positive control for the cotransfection step. This transfer vector has the E. coli beta-glucuronidase (GUS) gene cloned downstream of its polyhedrin promoter. Recombination of pBacPAK8-GUS with the BacPAK6 DNA digest generates recombinant viruses that express beta-glucuronidase. Expression of GUS can be detected by generation of a blue color from the chromogenic GUS substrate X-GLUC. More Less

avivasysbioVytas P. Ambutas- 董事会主席

Ambutas先生是一家多元化的工业控股公司National Material LP的总法律顾问，秘书兼法律和行政副总裁。Ambutas先生是Tang Industries，Inc.的总法律顾问，Tang Industries，Inc.是一家多元化控股公司，包括工业，制药，家具和房地产控股公司，并担任高强度碳素钢加工商Nacme Steel Processing，LLC的董事，以及Key Colony，Inc.，果汁产品的制造商和分销商。Ambutas先生从1980年至1992年曾从事私人执业。Ambutas先生拥有芝加哥洛约拉法学院的法学博士学位和西密歇根大学的学士学位。

朱利安- 董事会成员

朱利安·袁（Julian Yuan）是联合创始人兼董事会成员。袁先生还是英杰华生物科技公司的联合创始人和董事会成员。袁先生自1983年成立以来，一直担任Progressive Group Inc.（PGI）的总裁兼首席执行官。PGI是其中之一。台湾最大的医疗器械经销商。袁先生是一位企业家，在生物科学产品，医疗设备和仪器的管理和营销方面拥有22年的经验。袁先生于1979年在台湾中原大学获得化学学士学位。

Patrick Liang- 董事会成员

梁先生现任唐实业有限公司中国业务董事总经理兼唐投资集团（中国）有限公司总裁。之前，梁先生曾任中国材料有限公司总经理，SKD中国副总裁，以及Irvine Networks业务开发副总裁兼SCA咨询管理顾问。梁先生拥有芝加哥大学布斯商学院的工商管理硕士学位和双学位，并拥有西北大学的经济学学士学位和国际研究学士学位。